C1 Metabolism and Photorespiration of Ficus deltoidea based on Peptide Mass Fingerprinting Approach
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Metabolites Profiling Laboratory, Institute of Bioproduct Development, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor Bahru, Johor, MALAYSIA
Department of Bioprocess and Polymer Engineering, Faculty of Chemical and Energy Engineering, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor Bahru, Johor, MALAYSIA
Department of Biotechnology and Medical Engineering, Faculty of Biosciences & Medical Engineering, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor Bahru, Johor, MALAYSIA
Publish date: 2018-07-14
Eurasian J Anal Chem 2018;13(5):em41
Ficus deltoidea is a popular herbal plant as ethnomedicine, especially from its leaves. The decoction of leaves is used as tonic to regain energy, strengthen uterus, improve blood circulation, treat diabetes, gout, hypertension and also to reduce water in lung disease. Therefore, the plant physiology including its photorespiration mechanism is of great importance to understand its biological properties. Plant proteins are building blocks of many bioactive secondary metabolites. The present study extracted the plant proteins using Tris-buffered phenol technique, and then crude proteins were separated by gel electrophoresis prior to peptide identification using LC-QTOF MS. The identified proteins were used to explain the C1-metabolism and photorespiration in F. deltoidea. Mass spectra of peptides were found to match 229 proteins, and 9 of them were strongly related to C1-metabolism. The proteins such as pentatricopeptide repeat protein, tetratricopeptide repeat protein, 5,10-methylenetetrahydrofolate dehydrogenase:5,10-methenyltetrahydrofolate cyclohydrolase and folylpolyglutamate synthase are essential in photorespiratory cycle. The detection of the proteins suggests that F. deltoidea perform photorespiration via C1-THF synthase/SHMT pathway which is the alternative photorespiratory pathway. The findings of this study could be used to explain the production of bioactive metabolites in F. deltoidea. This is also the first report to reveal the C1-metabolism and photorespiration in F. deltoidea.
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