Isolation and Identification of Flavonoids in Iraqi Rheum ribes Root
More details
Hide details
Department of Biology, College of Education, Samara University, Samara, Salah Al Deen, IRAQ
Department of Chemistry, College of Education, Samara University, Samara, Salah Al Deen, IRAQ
Department of Biology, College of Education for Pure Sciences, University of Tikrit, Salah Al Deen, IRAQ
Publish date: 2018-10-02
Eurasian J Anal Chem 2018;13(5):em66
The aim of the study was isolate and identify the flavonoids in Iraqi Rheum ribes root, the results obtained from the HPLC analysis indicate that the Iraqi Rheum ribes root contain different types and concentration of flavonoids which include 29.9056 μg/g aloe-emodin, 252.880 μg/g emodin, 76.8493 μg/g chrysophanol, 56.1317 μg/g physician and 49.2098 μg/g rutin, while the types of flavonoids in the isolated extract of flavonoids from the Iraqi Rheum ribes root were 146.096 μg/g aleo-emodin, 37.174 μg/g emodin, 11.525 μg/g chrysophanol, 20.010 μg/g physcion, 30.271 μg/g rutin. The identification the all types of flavonoids in isolated flavonoids from the Iraqi Rheum ribes roots as compared with the flavonoids in the crude roots refers to the efficiency of the method for isolation of flavonoids from the roots of plant.
1. Kayser AF, Kidderlen SL, Croft SL. Natural products as potential anti-parasitic drugs Acta Trop. 2000;77:307-14.
2. Raskin I, Ribnicky DM, Komarnytsky S, Ilic N, Poulev A, Borinker N, Moreno DA, Ripoll C, Yakoby N. Plants and human health in the twenty-first century Trends Biotechnol. 2002;20:522-31.
3. Fernandez SP, Wasowski C, Loscalzo LM, et al. Central nervous system depressant action of flavonoid glycosides. Eur. J. Pharm. 2006;539:168-76.
4. Heim KE, Tagliaferro AR, Bobliya DJ. Flavonoids antioxidants: Chemistry, metabolism and structure-activity relationships. J. Nutr. Biochem. 2002;13:572-84.
5. Cushnie TPT, Lamb AJ. Antimicrobial activity of flavonoids. Int. J. Antimicrob. Agents 2005;26:343-56.
6. Harleen KS, Bimlesh K, Sunil P, Prashant T, Manoj S, Pardeep S. A Review of Phytochemistry and Pharmacology of Flavonoids. Internationale Pharmaceutica Sciencia 2011;1(1):25-41.
7. Tapas AR, Sakarkar DM, Kakde RB. Flavonoids as nutraceuticals: A Review. Trop. J. Pharm. Res. 2008;7:1089-99.
8. Narayana KR, Reddy SR, Chaluvadi MR, Krishna DR. Bioflavonoids classification, pharmacological, biochemical effects and therapeutic potential. Indian J. Pharmacol. 2001;33:2-16.
9. Havsteen BH. The biochemistry and medical significance of the flavonoids. Pharmacol. Ther. 2002;96:167-92.
10. Sriram PG, Subramanian S. Fisetin a bioflavonoid ameliorates hyperglycaemia in streptozotocin-induced experimental diabetes in rats. Int. J. Pharm. Sci. Rev. Res. 2011;6:68-74.
11. Davis PH. Flora of Turkey and East Aegean Islands. Cullen Journal. 1967;2:268–69.
12. Tuzlaci E, Mericli AH. Some studies on Isgin (Rheum ribes) and its distribution in Turkey. In: Baser KCH, ed.Proceedings of the IX Symposium on Plant Drugs. Eskisehir: Publication of Anadolu University, 1992:336–41.
13. Adham AN, Naqishbandi AM. HPLC analysis and antidiabetic effect of Rheum ribes root in type 2 diabetic patients. Zanco J. Med. Sci. 2015;19(2):366-72.
14. Alaadin AM, Al-Khateeb EH, Jager AK. Antibacterial Activity of the Iraqi Rheum ribes Root. Pharmaceutical Biology 2007. 45(9):688–90.
15. Abdulla KK, Taha EM, Rahim SM. Phenolic profile, antioxidant, and antibacterial effects of ethanol and aqueous extracts of Rheum ribes L. roots. Der Pharmacia Lettre. 2015;7(4):26-30.
16. Boubekri N, Belloum Z, Boukaabache R, et al. In vivo anti-inflammatory and in vitro antioxidant activities of Genista quadriflora Munby extracts. Der Pharmacia Lettre. 2014;6(1):1-7.
17. Chen JJ, Li XR, Fang X. Purification of total flavones from Morus alba L. by macroporous adsorbents and kinetic model for the process. J. Zhejiang Univ. Medical Sciences. 2006;35:219-23.
18. Oyaizu M. Studies on product of browning reaction prepared from glucose amine. Jpn. J. Nutr. 1986;44:307-15.
19. Suares B, Palacios N, Rodriguez R. Liquid chromatographic method for quantifying polyphenols in ciders by direct injection. J. of Chromatography A. 2005;1066:105-10.
20. Penarrieta JM, Alvarado JA, Bergenstahl B, Akesson B. Spectrophotometric methods for the measurement of total phenolic compounds and total flavonoids in foods. Revista Bioliviana de Quimica 2007;24(1):5-9.
21. Ibrahim EA, Abou Baker DH, El-Baz FK. Anti-Inflammatory and Antioxidant Activities of Rhubarb Roots Extract. Int. J. Pharm. Sci. Rev. Res.2016;39(2):93-9.
22. Naqishbandi AM, Jäger AK, Al-Khateeb EH. A Comparative Qualitative and Quantitative Study of Anthraquinone Derivatives in the Roots of Rheum ribes and Rheum emodi by HPLC. Iraqi J Pharm Sci, 2009;18(Suppl):60-4.
23. Mericli AH, Tuzlaci E. Constituents of Rheum ribes. Fitoterapia. 1990;367:75.
24. Al-Salihi FG, Majeed AH, Hameed RR. Hypolipidemic effect of date palm pollen and isolated flavonoids in sera of adult male rabbits. Kerbala Journal of Pharmaceutical Sciences. 2013;5:34-45.