A 1H-NMR based Method for Quantifying Creatinine in Urine Samples from Human Intervention Studies
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Department of Chemistry, Division of Food Chemistry and Toxicology, University of Kaiserslautern, Erwin-Schroedinger-Str. 52, D-67663 Kaiserslautern, GERMANY
Department of Chemistry, Division of Inorganic Chemistry, University of Kaiserslautern, Erwin-Schroedinger-Str. 52, D-67663 Kaiserslautern, GERMANY
Publication date: 2018-09-07
Eurasian J Anal Chem 2018;13(5):em51
To overcome problems arising from the coloration of urine samples and the limitations of the picric acid method when determining creatinine in urine, we have established an alternative method using 1H-NMR. The method is practically straight forward, provides good recoveries of 105 ± 2%, and a linear regression yielded an R2 value of 1. The method revealed a similar precision to the standard procedure based on the Jaffé reaction for urine samples without interfering components.
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