RESEARCH PAPER
A 1H-NMR based Method for Quantifying Creatinine in Urine Samples from Human Intervention Studies
 
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1
Department of Chemistry, Division of Food Chemistry and Toxicology, University of Kaiserslautern, Erwin-Schroedinger-Str. 52, D-67663 Kaiserslautern, GERMANY
2
Department of Chemistry, Division of Inorganic Chemistry, University of Kaiserslautern, Erwin-Schroedinger-Str. 52, D-67663 Kaiserslautern, GERMANY
Publish date: 2018-09-07
 
Eurasian J Anal Chem 2018;13(5):em51
KEYWORDS:
ABSTRACT:
To overcome problems arising from the coloration of urine samples and the limitations of the picric acid method when determining creatinine in urine, we have established an alternative method using 1H-NMR. The method is practically straight forward, provides good recoveries of 105 ± 2%, and a linear regression yielded an R2 value of 1. The method revealed a similar precision to the standard procedure based on the Jaffé reaction for urine samples without interfering components.
 
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