A Quick GC/MS Method Correlated with LC/MS/MS for the Identification of Medicinal Natural Products in Convolvulus Arvensis: An Injury Healing Plant
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Department of Chemistry and Chemical Technology, Tafila Technical University, Tafila, Jordan
Department of Chemistry, Faculty of Science, Al-Balqa Applied University, Al-Salt, Jordan
Publish date: 2017-10-05
Eurasian J Anal Chem 2015;10(3):137–149
The identification of the natural products in methanolic extracts of Convolvulus arvensis plant parts was carried out by using GC/MS and LC/MS/MS chromatographic systems. The study was performed on both fresh and dry samples. The analysis reveals that many compounds are presented in the extracts. Some were reported and characterized earlier in several other plants including their biological activities. Each part of the plant was treated similarly in all steps. The GC/MS chromatograms present peaks that are major (peak area/total peaks area>0.2%). In the leaves extract, seventeen compounds were detected as individual, sharp, well resolved and readily quantified peaks. Four peaks are major and identified to be Neophytadiene, hexadecanamide, 9-octadecanamide and 1,2-benzendicarboxylic acid. In stems extract, thirteen compounds were observed, four peaks are major: hexadecanamide, 9-octadecanamide, 1,2-benzendicarboxylic acid and stigment-5-en-3-ol. In roots extract, twelve compounds were detected, three peaks are major: hexadecanamide, 9-octadecanamide and 1,2-benzendicarboxylic acid. The dry extract contains 25 compounds, eight compounds are major and identified to be Neophytadiene, stearic acid, hexadecanamide, 9-octadecanamide, 1,2-benzendicarboxylic acid, Vitamin E, stigment-5-en-3-ol and 5-beta-pregn-7-en-3,20-dione. The LC/MS/MS measurements were performed and the results of the two chromatographic methods were correlated and found to be consistent. The major compounds are detected in the two chromatographic methods. The dry and fresh plant was traditionally examined to be used for treatment of different ailments. This study was performed because the fresh and dry Convolvulus arvensis were used for the treatment of skin cuts or wounds, to stop bleeding and promote quick injury healing.
Khalid Abu-Shandi   
Department of Chemistry and Chemical Technology, Tafila Technical University, Tafila, Jordan
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