A Simple and Environmentally Friendly Reflectometric Method for the Rapid Quantitative Analysis of Captopril in Pharmaceutical Formulations
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Centro de Ciências Sociais, Saúde e Tecnologia – UFMA, CEP 65900-000, Imperatriz, MA, Brazil
Instituto de Química – UNESP, P.O. Box 355, CEP 14801-970, Araraquara, SP, Brazil
Paulo Roberto S. Ribeiro   

Centro de Ciências Sociais, Saúde e Tecnologia – UFMA, CEP 65900-000, Imperatriz, MA, Brazil
Publication date: 2017-10-15
Eurasian J Anal Chem 2011;6(2):91–103
This paper describes a simple, environmentally friendly and rapid quantitative spot test procedure for the determination of captopril (CPT) in bulk drug and in pharmaceutical formulations by using diffuse reflectance spectroscopy. The proposed method is based on the reflectance measurements of the orange compound ( λmax 490 nm) produced by the spot test reaction between CPT and p-chloranil (CL). Under optimal conditions, calibration curves were obtained for CPT by plotting the optical density of the reflectance signal (AR) vs. the log of the mol L-1 concentration, from 6.91x10-3 to 1.17x10-1, with a good coefficient of determination (R2 = 0.9992). The common excipients used as additives in pharmaceuticals do not interfere in the proposed method. The method was applied to determine CPT in commercial pharmaceutical formulations. The results obtained by the proposed method are compared favorably with those obtained by an official procedure at 95% confidence level. The method validation results showed that the sensitivity and selectivity of the methods were adequated for drug monitoring in industrial quality control laboratories.
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