A Simple and Rapid Spectrophotometric Method for the Determination of Artesunate in Pharmaceuticals
 
More details
Hide details
1
Department of Post Graduate Studies and Research in Chemistry, Mangalore University, Mangalagangothri, Karnataka, 574-199, India
Publish date: 2017-10-06
 
Eurasian J Anal Chem 2009;4(1):119–126
KEYWORDS
ABSTRACT
Simple and rapid spectrophotometric method for the determination of artesunate is described. The method is based on the reaction of H2O2, generated by the cleavage of endoperoxide linkage of artesunate and its reaction with potassium iodide to liberate iodine. The liberated iodine bleaches the red colored Safranin O to colorless species and is measured at 521 nm while it oxidizes colorless Variamine blue to violet colored species and is measured at 556 nm. The optimum reaction conditions and other analytical parameters were evaluated. The statistical evaluation of the method is examined by determining intra-day and inter-day precision. The method has been successfully applied to both pure and tablet dosage forms.
 
REFERENCES (21)
1.
Mukherjee T (1991) Antimalarial herbal drugs. A review. Fitoterapia, 62:197.
 
2.
Liu J-M, Ni M-Y, Fan J-F, Tu Y-Y, Wu Z-H, Wu Y-L and Zhou W–S (1979) Structure and reactions of arteannuin, Acta Chimica Sinica, 37:129.
 
3.
Li Y, Yu P-L, Chen Y-X, Li L-Q, Gai Y Z, Wang D S and Zheng Y- P (1981) Studies on analogs of artemisinin.I. Synthesis of ethers, carboxylic esters and carbonates of dihydroartemisinin, Acta Pharmaceutica Sinica, 16: 429.
 
4.
China Co-operative Research Group on Qinghaosu and its Derivatives as Antimalarials (1982) J. Tradit. Chin. Med. 2: 9.
 
5.
Jeremi´C D, Joki´C A, Behbud A and Stefanovi´c M (1973) A new type of sesqiterpene lactone isolated from artemisia annua L. arteannuin B. Tetrahedron Lett. 14: 3039.
 
6.
Lin A J, Klayman D L and Milhous W K (1987) Antimalarial activity of new watersoluble dihydroartemisinin derivatives. J. Med. Chem. 30: 2147.
 
7.
Brossi A, Venugopalan B, Dominguez G L, Yeh H J C, Flippen-Anderson J L, Buchs P, Luo X- D, Milhous W and Peters W (1988) Arteether, a new antimalarial drug: Synthesis and antimalarial properties. J. Med. Chem. 31:645.
 
8.
Klayman D L (1985) Qinghaosu (artemisinin): an antimalarial drug from China Science 228: 1049.
 
9.
Butler A R and Wu Y-L (1992) Artemisinin (qinghaosu): a new type of antimalarial drug. Chem. Soc. Rev. 21: 85.
 
10.
Woerdenbag H J, Pras N, Uden W V, Wallaart T E, Beekman A C and Lugt C B (1994) Progress in the research of artemisinin-related antimalarials: an update. Pharm. World Sci. 16: 169.
 
11.
Meshnick S R, Taylor T E and Kamchonwongpaisan S (1996) Artemisinin and the antimalarial endoperoxides from herbal remedy to targeted chemotherapy. Microbiol. Rev. 60: 301.
 
12.
Haynes R K (2006) Artemisinin to new artemisinin antimalarials: Biosynthesis, extraction, old and new derivatives, stereochemistry and medicinal chemistry requirements. Current Topics in Medicinal Chemistry 6: 509.
 
13.
De Vries P J and Dien T K (1996) Clinical pharmacology and therapeutic potential of artemisinin and its derivatives in the treatment of malaria. Drugs, 52: 818.
 
14.
Gabriels M and Plaizier-Vercammen J (2004) Development of a reversed phase thinlayer chromatographic method for artemisinin and its derivatives. J. Chromatogr. Sci. 42: 341.
 
15.
Naik H, Murry D J, Kirsch L E and Fleckenstein L (2005) Development and validation of a high-performance liquid chromatography–mass spectroscopy assay for determination of artesunate and dihydroartemisinin in human plasma J. Chromatogr. B Analyt Technol. Biomed. Life Sci. 816: 233.
 
16.
Rajanikanth M, Madhusudhanan K P and Gupta R C (2003) An HPLC-MS method for simultaneous estimation of alpha, beta arteether and its metabolite dihydroartemisinin, in rat plasma for application to pharmacokinetic study. Biomed. Chromatogr. 17: 440.
 
17.
Na-Bangchang K, Congpuong K, Hung L N, Molunto P and Karbwang J (1998) Simple high-performance liquid chromatographic method with electrochemical detection for the simultaneous determination of artesunate and dihydroartemisinin in biological fluids. J. Chromatogr. B Biomed. Sci. Appl. 708: 201.
 
18.
Taylor R B, Awad M I, Reid R G, and Moody R R (2000) Determination of sodium artesunate in plasma using ion-pairing high-performance liquid. J. Chromatogr. B Biomed. Sci. Appl. 744: 415.
 
19.
van Quekelberghe, Soomro, S A, Cordonnier J A and Jansen F H (2008) Optimization of an LC-MS method for the determination of artesunate and dihydroartemisinin plasma levels using liquid-liquid extraction. J. Anal. Toxicol. 32:133.
 
20.
Lindegardh N, Dondorp A M, Singhasivanon P, White N J and Day N P J (2007) Validation and application of a liquid chromatographic-mass spectrometric method for determination of artesunate in pharmaceutical samples. J.Pharm.Biomed.Anal. 45:149.
 
21.
Agarwal S, Ali A and Ahuja S (2007) HPTLC determination of artesunate as bulk drug and in pharmaceutical formulations. Indian J. Pharm. Sci., 69: 841.
 
eISSN:1306-3057