A Simple and Validated HPTLC Method of Evaluation for Quetiapine fumarate in Oral Solid Dosage Form
 
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1
Institute of Pharmacology, Madras Medical College, Chennai 600003, India
2
TIFAC CORE HD, JSS College of Pharmacy, Rocklands, Ootacamund 643001, India
CORRESPONDING AUTHOR
Kaliaperumal Krishnaraj   

TIFAC CORE HD, JSS College of Pharmacy, Rocklands, Ootacamund 643001, India
Publication date: 2017-10-08
 
Eurasian J Anal Chem 2010;5(3):246–253
 
KEYWORDS
ABSTRACT
A simple, sensitive, precise and rapid high performance thin layer chromatographic method of analysis for quetiapine fumarate in pharmaceutical formulation was developed and validated. The method was employed in thin layer chromatographic aluminum plates precoated with silica gel 60F-254 as the stationary phase. The solvent system consist of Toluene: Ethyl acetate: Diethyl amine (5:3:2, v/v/v) as the mobile phase. Densitometric analysis of quetiapine fumarate was carried out in the absorbance mode at 291 nm. The system was found to give compact spots for quetiapine fumarate (Rf value of 0.54). The linear regression analysis data for the calibration plots showed good linear relationship with r2 = 0.9915 in the concentration range 25-225 ng per spot. The method was validated for precision, accuracy, recovery and sensitivity. The method has been successfully applied in the analysis of oral solid dosage formulation.
 
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