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RESEARCH PAPER
A Study of Voltammetric Behavior of Spermine and Its Application for Estimation of Polyamine Oxidase Activity
| 1 | College of Science, University of Mosul, IRAQ |
| 2 | College of Education for Girls, University of Mosul, IRAQ |
Online publication date: 2018-09-30
Publication date: 2018-09-30
Eurasian J Anal Chem 2018;13(5):em63
KEYWORDS
ABSTRACT
Here, we present details about the voltammetric behavior of spermine (spm), showing a well-defined current peak at -0.45 V, compared to AgCl, as a reference electrode, using square wave voltammetry (SWV). Under optimal conditions, the voltammetric current is proportional to the concentration of spm, over the concentration range of 4.99 ×10-8 –7.98×10-7 M, with a correlation coefficient of R= 0.994 and a limit of detection of 0.7 ×10-8 M. Repeatability was tested for 9.99×10-7 M spm (n = 6), the current was 41 nA, and standard deviation (SD) was ±1.5 nA. In addition, the SD for instrument error was 1.7 nA. The proposed method was successfully applied to examine the polyamine oxidase (PAO) activity in cow and sheep’s milk. A comparative study between SWV electrochemical and spectroscopic methods showed a good correlation between the two methods, indicating that the proposed method can be used as an alternative analytical technique.
REFERENCES (29)
2.
Morgan L. Polyamine oxidases. Biochem Soc Trans 1985;13:322-6. https://doi.org/10.1042/bst013....
3.
Morgan L IG. Polyamine-polyamine oxidase interaction: part of maternal protective mechanism against fetal rejection. Br Med J. 1980;280(6227):1295-7. https://doi.org/10.1136/bmj.28....
4.
Moller K TT, Fink N. Bovine colostrum is superior to enriched formulas in stimulating intestinal function and necrotizing enterocolitis resistance in preterm pigs. Br J Nutr. 2011;105(1):44-53. https://doi.org/10.1017/S00071....
5.
Zarban A TF, Chahkandi T, et al. Pattern of total antioxidant capacity in human milk during the course of lactation. Iran J Pediatr. 2007;17 (suppl 1):34-40.
6.
DML M. Polyamine oxidase – enzyme of unknown function. Biochem Soc Trans. 1998;26:586- 90. https://doi.org/10.1042/bst026....
7.
Pollack PF. KOaNK. Polymine in human and rat milk and in infant formulas. Am J clin Nutr 1992;56:371-5. https://doi.org/10.1093/ajcn/5....
8.
H FKaW. Polyamine oxidase activity in a human colonic carcinoma cell line. Biochem Sco trans. 1990;18:1225. https://doi.org/10.1042/bst018....
9.
Tomitori H, Usui T, Saeki N, Ueda S, Kase H, Nishimura K, et al. Polyamine oxidase and acrolein as novel biochemical markers for diagnosis of cerebral stroke. Stroke. 2005;36(12):2609-13. https://doi.org/10.1161/01.STR....
10.
SM AK. Occurrence and properties of polyamine oxidase isoenzymes in the milk and cerebrospinal fluid. Iraq: Coll Edu Mosul Uni; 2000.
11.
Dahel KA A-SNaFK. Polyamine oxidase activity in sera of depressed and schizophrenic patients after ECT treatment. Neurochem Res 2001;26(4):415-8. https://doi.org/10.1023/A:1010....
12.
Flayeh K. Spermidine oxidase activity in serum of normal and schizophrenic subject. Clin Chem. 1988;412:401-3.
13.
Royo M, Fitzpatrick PF. Mechanistic studies of mouse polyamine oxidase with N1,N12-bisethylspermine as a substrate. Biochemistry. 2005;44(18):7079-84. https://doi.org/10.1021/bi0503....
14.
Nag S, Saha K, Choudhuri MA. A rapid and sensitive assay method for measuring amine oxidase based on hydrogen peroxide-titanium complex formation. Plant Sci. 2000;157(2):157-63. https://doi.org/10.1016/S0168-....
15.
Igarashi K, Kashiwagi K, Hamasaki H, Miura A, Kakegawa T, Hirose S, et al. Formation of a compensatory polyamine by Escherichia coli polyamine-requiring mutants during growth in the absence of polyamines. J Bacteriol. 1986;166(1):128-34. https://doi.org/10.1128/jb.166....
16.
Bagotsky V. Fundamentals of Electrochemistry. 2nd ed. New Jersey: John Wiley & Sons, Inc. Hoboken; 2006.
17.
Al kasir RS, Al-Wahab H. A, Sulaiman S.T. Measurement of Glutam ate-Oxalacetate Transami nase Activity in Human Blood Serum Using Differential Pulse Anodic Stripping Voltammetry. Jou Raf Sci. 2010;21(4):11-8.
18.
A.M A-L. Electro - biochemical Study of Some Biogenic Amines and Amine Oxidases Using Different Modified Electrodes-Construction of Nano Composite Biosenser Iraq: Coll Sci Mosul Uni; 2011.
19.
Sulaiman S.T. NM. Differential pulse polarographic behaviors of p- nitrosodimethylaniline: Application for microdetermination of NADH. Microchemical Journal. 1985;31(1):37-43. https://doi.org/10.1016/0026-2....
20.
Suliman S.T RFH. A Differential- Pulse Polarographic Determination of Doxycycline in Serum and Urine. Raf Jour Sci. 2008;19(1):52-8.
22.
Zuman P RJ. Polographic Studies Of Adsorption at Mercury Electrode Surfaces. In Encyclopedia of Surface and Colloid Science: Marcel Dekker Inc.; 2002. p. 4143- 61.
23.
Christopher M. Riley TWR, editor. Development and Validation of Analytical Methods. 1st ed1996.
24.
Swartz ME, Krull, I.S. Analytical Development and Validation. New York: Marcel Dekker Inc; 1997.
25.
Feng X, Chen S, Xu Y, Du W, Luo Q, Liu BF. Separation and determination of biogenic amines in fish using MEKC with novel multiphoton excitation fluorescence detection. J Sep Sci. 2008;31(5):824-8. https://doi.org/10.1002/jssc.2....
26.
Miyachi K MK, Yamaguchi T, Tominaga H, Kamino SH and Fujita Y. Spectrophotometric Determination of Spermine and Related Compounds Using o- Hydroxyhydroquinonephthalein and Manganese (II). Anal Sci 2007;23:1103-7. https://doi.org/10.2116/analsc....
27.
Ozdestan O, Uren A. A method for benzoyl chloride derivatization of biogenic amines for high performance liquid chromatography. Talanta. 2009;78(4-5):1321-6. https://doi.org/10.1016/j.tala....
28.
Daniela Giorgio ADT, Salvatore Claps. Oligosaccharides, polyamines and sphingolipids in ruminant milk. Small Ruminant Research 2018;160:23-30. https://doi.org/10.1016/j.smal....
29.
Loser C. Polyamines in human and animal milk. British Journal of Nutrition. 2000;84:S55-S8. https://doi.org/10.1017/S00071....
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