RESEARCH PAPER
Antibacterial and Antioxidant Activity of the pigment produced by Monascus purpureus
 
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Online publication date: 2018-05-25
Publication date: 2018-04-30
 
Eurasian J Anal Chem 2018;13(2):emEJAC201813
 
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ABSTRACT
Polyketide pigments were isolated and purified from Monascus purpureus (MTCC 1090). The quantity of pigment was higher in intracellular than extra cellular. The pigment was produced by submerged and solid state fermentation. Pigment accumulation was observed on 3rd day during solid state fermentation and the SEM spore morphology reveals that the size of spore was 10µm long. The 3 different pigments Red, orange, yellow were seperated from the methanol extract of intracellular pigment. The antimicrobial activity of isolated pigment were done towards 4 bacteria namely S.aureus, E. coli, Klesiella sp., Providencia sp. Out of four test pathogen .three were sensitive for red and all are sensitive for crude pigment but not against purified yellow and orange. The antioxidant activity of orange pigment isolated from M. purpureus was confirmed by FRAP assay and compared with ascorbic acid. The reducing power of the extract might be due to their hydrogen‐donating ability. Microbial pigments can be an alternative to the use of synthetic compounds in food and pharmaceutical technology. Further it was also observed that these pigments were non toxic confirmed by in vivo larvicidal assay. The present study concludes pigments from Monascus purpuresus holding various pigments having various potent biological activities like antibacterial, and antioxidant effect without any side effect.
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