Approaches Development and Validation of RP-HPLC Method for Estimation of Glimepiride in Rat Plasma-Application to Pharmacokinetic Studies
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Department of Pharmacology, P. Wadhwani College of Pharmacy, Yavatmal-445001, Maharashtra, India
Department of Quality Assurance, P. Wadhwani college of Pharmacy, Yavatmal, Maharashtra, India
Department of Pharmaceutical Chemistry, P. Wadhwani college of Pharmacy, Yavatmal, Maharashtra, INDIA
Department of Pharmaceutical Sciences, Birla Institute of Technology, Mesra, Ranchi, Jharkhand, India
Publication date: 2017-10-27
Eurasian J Anal Chem 2016;11(2):55–61
A simple and sensitive method was developed for estimation of Glimepiride (GLIM) in rat Plasma by reverse phase high performance liquid chromatography (RP-HPLC). The drug samples were extracted by liquid-liquid extraction with 300 µL of acetonitrile and 5 mL of diethyl ether. Chromatographic separation was achieved on C18 column using methanol: water (85:15 v/v) as mobile phase at a flow rate of 1ml/min and UV detection at 230 nm. The retention time of GLIM was found to be 2.5 min. The developed method was validated for accuracy, precision, linearity and recovery. Linearity studies were found to be acceptable over the range of 100 – 6000 ng/mL. The method was successfully applied for the analysis of rat plasma sample for application in pharmacokinetic study, bioavailability.
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