Assay Determination of Tranexamic acid in Pharmaceutical Dosage Form (Tablet) Using HPLC and ELS Detector
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Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad- 431004 (MS), India
Wockhardt Research Centre, Aurangabad-431210 (MS), India
Publish date: 2017-10-08
Eurasian J Anal Chem 2010;5(3):204–211
A simple, rapid, and precise method is developed for the assay determination of tranexamic acid (Tr) in pharmaceutical dosage form. The method was developed with HPLC, ELS detector and Alltima cyano 100A (250 x 4.6 mm, 5µ) column, the mobile phase composed of aqueous solution of 0.05% v/v trifluoroacetic acid and acetonitrile (60:40 v/v, pH 2.85). The instrumental settings are flow rate of 0.5 mL min-1, column temperature at 300C and detector drift tube temperature set at 970C, a detector nitrogen gas flow set at 2.6 SLPM, gain 1 and 5 µL sample injection volume. Theoretical plates were 20007. Tailing factor was 1.38. The described method shows excellent linearity over a range of 50 to 150% of assay concentration. The correlation coefficient was 0.9987. The relative standard deviation of six measurements for peak area and retention time less than 2% and 0.5% respectively. The proposed method was found to be suitable and accurate for assay determination of Tr in pharmaceutical preparations.
Devanand B. Shinde   
Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad- 431004 (MS), India
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