RESEARCH PAPER
C1 Metabolism and Photorespiration of Ficus deltoidea based on Peptide Mass Fingerprinting Approach
 
More details
Hide details
1
Metabolites Profiling Laboratory, Institute of Bioproduct Development, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor Bahru, Johor, MALAYSIA
2
Department of Bioprocess and Polymer Engineering, Faculty of Chemical and Energy Engineering, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor Bahru, Johor, MALAYSIA
3
Department of Biotechnology and Medical Engineering, Faculty of Biosciences & Medical Engineering, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor Bahru, Johor, MALAYSIA
Publish date: 2018-07-14
 
Eurasian J Anal Chem 2018;13(5):em41
KEYWORDS:
ABSTRACT:
Ficus deltoidea is a popular herbal plant as ethnomedicine, especially from its leaves. The decoction of leaves is used as tonic to regain energy, strengthen uterus, improve blood circulation, treat diabetes, gout, hypertension and also to reduce water in lung disease. Therefore, the plant physiology including its photorespiration mechanism is of great importance to understand its biological properties. Plant proteins are building blocks of many bioactive secondary metabolites. The present study extracted the plant proteins using Tris-buffered phenol technique, and then crude proteins were separated by gel electrophoresis prior to peptide identification using LC-QTOF MS. The identified proteins were used to explain the C1-metabolism and photorespiration in F. deltoidea. Mass spectra of peptides were found to match 229 proteins, and 9 of them were strongly related to C1-metabolism. The proteins such as pentatricopeptide repeat protein, tetratricopeptide repeat protein, 5,10-methylenetetrahydrofolate dehydrogenase:5,10-methenyltetrahydrofolate cyclohydrolase and folylpolyglutamate synthase are essential in photorespiratory cycle. The detection of the proteins suggests that F. deltoidea perform photorespiration via C1-THF synthase/SHMT pathway which is the alternative photorespiratory pathway. The findings of this study could be used to explain the production of bioactive metabolites in F. deltoidea. This is also the first report to reveal the C1-metabolism and photorespiration in F. deltoidea.
 
REFERENCES (33):
1. Fatihah HNN, Mat N, Zaimah ARN, Zuhailah MN, Norhaslinda H, Khairil M, Ghani AY, Ali AM. Morphological Phylogenetic Analysis of Seven Varieties of Ficus deltoidea Jack from the Malay Peninsula of Malaysia. PLoS One 2012;7:1–7. https://doi.org/10.1371/journa....
2. Ramamurthy S, Kumarappan C, Dharmalingam SR, Sangeh JK. Phytochemical , Pharmacological and Toxicological Properties of Ficus deltoidea : A Review of a Recent Research. 2014;4:2357–2371. Retrieved from http://www.sciencedomain.org/r....
3. Ong HC, Zuki RM, Milow P. Traditional Knowledge of Medicinal Plants Among the Malay Villagers in Kampung Mak Kemas, Terengganu, Malaysia. Stud Ethno-Medicine 2011;5:175–185. https://doi.org/10.1080/097350....
4. Farhana MMH, Fauzi PA, Lim HF. Market Potential for Mas Cotek (Ficus deltoidea) Products in Selected States in Peninsular Malaysia. For. Res. Inst. Malaysia 2007:132–135.
5. Deonikar P, Kothandaram S, Mohan M, Kollin C, Konecky P, Olovyanniko R, Zamore Z, Carey B, Ayyadurai VAS. Discovery of Key Molecular Pathways of C1 Metabolism and Formaldehyde Detoxification in Maize through a Systematic Bioinformatics Literature Review. Agric Sci 2015;6:571–585. https://doi.org/10.4236/as.201....
6. Hanson AD, Gage DA, Shachar-Hill Y. Plant One-Carbon Metabolism and its Engineering. Plant Cell 2000;5:206–213. https://doi.org/10.1016/S1360-....
7. Hanson AD, Roje S. One Carbon Metabolism in Higher Plant. Annu Rev Plant Physiol Plant Mol Biol 2001;52:119–144. https://doi.org/10.1146/annure....
8. Peterhansel C, Horst I, Niessen M, Blume C, Kebeish R, Kürkcüoglu S, Kreuzaler F. Photorespiration. Arabidopsis Book 2010;8:1–24. https://doi.org/10.1199/tab.01....
9. Wingler A, Quick WP, Bungard RA, Bailey KJ, Lea PJ, Leegood RC. The Role of Photorespiration During Drought Stress : An Analysis Utilizing Barley Mutants with Reduced Activities of Photorespiratory Enzymes. Plant, Cell Environ 1999;22:361–373. https://doi.org/10.1046/j.1365....
10. Bauwe H, Hagemann M, Fernie AR. Photorespiration: Players, Partners and Origin. Trends Plant Sci 2010;15:330–336. https://doi.org/10.1016/j.tpla....
11. Voss I, Sunil B, Scheibe R, Raghavendra AS. Emerging Concept for the Role of Photorespiration as an Important Part of Abiotic Stress Response. Plant Biol. 2013;15:713–722. https://doi.org/10.1111/j.1438....
12. Isaacson T, Damasceno CMB, Saravanan RS, He Y, Catalá C, Saladié M, Rose JK. Sample Extraction Techniques For Enhanced Proteomic Analysis of Plant Tissues. Nat Protoc 2006;1:769–774. https://doi.org/10.1038/nprot.....
13. Abdullah FI, Chua LS, Rahmat Z. Comparison of protein extraction methods for the leaves of Ficus deltoidea. J Fundam Appl Sci. 2017;9:908-924. https://doi.org/10.4314/jfas.v....
14. Canovas FM, Dumas‐Gaudot E, Recorbet G, Jorrin J, Mock HP, Rossignol M. Plant Proteome Analysis. Proteomics 2004;4:285–298. https://doi.org/10.1002/pmic.2....
15. Wang W, Tai F, Chen S. Optimizing Protein Extraction from Plant Tissues for Enhanced Proteomics Analysis. J Sep Sci 2008;31:2032–2039. https://doi.org/10.1002/jssc.2....
16. Gorelova V, Ambach L, Rébeillé F, Stove C. Folates in Plants : Research Advances and Progress in Crop Biofortification. Front Chem 2017;5:1–20. https://doi.org/10.3389/fchem.....
17. Ravanel S, Cherest H, Jabrin S, Grunwald D, Surdin-Kerjan Y, Douce R, Rébeillé F. Tetrahydrofolate Biosynthesis in Plants : Molecular and Functional Characterization of Dihydrofolate Synthetase and Three Isoforms of Folylpolyglutamate Synthetase in Arabidopsis thaliana. PNAS 2001;98:15360–15365. https://doi.org/10.1073/pnas.2....
18. Gambonnet B, Jabrin S, Ravanel S, Karan M, Douce R, Rébeillé F. Folate Distribution During Higher Plant Development. J Sci Food Agric 2001;81:835–841. https://doi.org/10.1002/jsfa.8....
19. Jabrin S, Ravanel S, Gambonnet B, Douce R, Rébeillé F. One-Carbon Metabolism in Plants. Regulation of Tetrahydrofolate Synthesis During Germination and Seedling Development. Plant Physiol 2003;131:1431–1439. https://doi.org/10.1104/pp.016....
20. Wingler A, Lea PJ, Leegood RC. Photorespiratory Metabolism of Glyoxylate and Formate in Glycine-Accumulating Mutants of Barley and Amaranthus edulis. Planta 1999;207:518–526. https://doi.org/10.1007/s00425....
21. Nour JM, Rabinowitzs JC. Isolation, Characterization, and Structural Organization of 10-Formyltetrahydrofolate Synthetase from Spinach Leaves. J Biol Chem 1991;266:18363–18369.
22. Chen L, Nargang FE, Edwin A. Isolation and Sequencing of a Plant cDNA Encoding a Bifunctional Methylenetetrahydrofolate Dehydrogenase : Methenyltetrahydrofolate Cyclohydrolase Protein. Pteridines 1999;10:171–177. https://doi.org/10.1515.
23. Chen L, Chan SY, Cossins EA. Distribution of Folate Derivatives and Enzymes for Synthesis of 1 O-Formyltetrahydrofolate in Cytosolic and Mitochondrial Fractions of Pea Leaves. Plant Physiol 1997;115:299–309. https://doi.org/10.1104/pp.115....
24. Barkan A, Small I. Pentatricopeptide Repeat Proteins in Plants. Annu Rev Plant Biol 2014;65:415–442. https://doi.org/10.1146/annure....
25. Schmitz-linneweber C, Small I. Pentatricopeptide Repeat Proteins : A Socket Set for Organelle Gene Expression. Trends Plant Sci 2008;152:663–670. https://doi.org/10.1016/j.tpla....
26. Zhang HD, Cui YL, Huang C, Yin QQ, Qin XM, Xu T, He XF, Zhang Y, Li ZR, Yang ZN. PPR Protein PDM1/SEL1 is Involved in RNA Editing and Splicing of Plastid Genes in Arabidopsis thaliana. Photosynth Res 2015;126:311–321. https://doi.org/10.1007/s11120....
27. Manna S. An Overview of Pentatricopeptide Repeat Proteins and their Applications. Biochimie 2015;113:93–99. https://doi.org/10.1016/j.bioc....
28. Blatch GL, La M. The Tetratricopeptide Repeat : A Structural Motif Mediating Protein-Protein Interactions. BioEssays 1999;21:932–939. https://doi.org/10.1002/(SICI)...<932::AID-IES5>3.0.CO;2-N.
29. Mirus O, Bionda T, Haeseler A Von, Schleiff E. Evolutionarily Evolved Discriminators in The 3-TPR Domain of the Toc64 Family Involved in Protein Translocation at the Outer Membrane of Chloroplasts and Mitochondria. J Mol Model 2009;15:971–982. https://doi.org/10.1007/s00894....
30. Haucke V, Horst M, Schatz G, Lithgow T. The Mas20p and Mas70p Subunits of the Protein Import Receptor of Yeast Mitochondria Interact via the Tetratricopeptide Repeat Motif in Mas20p: Evidence For a Single Hetero-Oligomeric Receptor. EMBO J 1996;15:1231.
31. Young JC, Hoogenraad NJ, Hartl FU. Molecular Chaperones Hsp90 and Hsp70 Deliver Preproteins to the Mitochondrial Import Receptor Tom70. Cell 2003;112:41–50. https://doi.org/10.1016/S0092-....
32. Yang C-S, Weiner H. Yeast Two-Hybrid Screening Identifies Binding Partners of Human Tom34 that have ATPase Activity and Form a Complex with Tom34 in the Cytosol. Arch Biochem Biophys 2002;400:105–110. https://doi.org/10.1006/abbi.2....
33. Ponting CP. Proteins of the Endoplasmic-Reticulum-Associated Degradation Pathway: Domain Detection and Function Prediction. Biochem J 2000;351:527–535. https://doi.org/10.1042/bj3510....
eISSN:1306-3057