0.60
CiteScore
0.186
SJR
0.447
SNIP
 
 

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE SIMULTANEOUS DETERMINATION OF AMILORIDE HYDROCHLORIDE AND FUROSEMIDE IN PURE AND PHARMACEUTICAL DOSAGE FORM

 
1
Sardar Patel College of pharmacy, Bakrol
Eurasian J Anal Chem 2017;12(4)
Publish date: 2017-01-04
KEYWORDS:
TOPICS:
ABSTRACT:
Background:
A simple, selective, rapid, precise and RP-HPLC method has been developed for the simultaneous estimation of Amiloride Hydrochloride and Furosemide from pharmaceutical formulation. column : Enable C18 (250x4.6) mm;5 µm , mobile phase: acetonitrile: Water(pH 4 using orthophosphoric acid) (70:30 v/v), flow rate : 1.0 ml/min. The retention times of Amiloride Hydrochloride-2.21 min. Furosemide- 7.60 min.The developed method was validated according to ICH guidelines for evaluation of accuracy, precision, linearity, LOD, LOQ and robustness.

Material and methods:
AML, FUR, Acetonitrile, water, orthophosphoric acid, RP-HPLC, Validation.

Results:
Linearity : 10-85(µg/ml) for AML, 80-685(µg/ml) for FUR. % RSD was found within the limit. % recovery and Assay range also found between the range.

Conclusions:
The proposed sensitive RP-HPLC method gives accurate and precise results.The proposed method was successfully applied to determination of these drugs in commercial tablets.

CORRESPONDING AUTHOR:
Jinal Natvarlal Tandel   
Sardar Patel College of pharmacy, Bakrol, Sardar patel college of pharmacy, Bakrol, Dist- Anand, 388001 Anand, India
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eISSN:1306-3057