Derivatization of Artesunate and Dihydroartemisinin for Colorimetric Analysis using p-dimethylaminobenzaldehyde
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Department of Pharmaceutical Chemistry, Faculty of Pharmacy University of Ibadan, Ibadan, Nigeria
Olajire A. Adegoke   

Department of Pharmaceutical Chemistry, Faculty of Pharmacy University of Ibadan, Ibadan, Nigeria
Publication date: 2017-10-15
Eurasian J Anal Chem 2011;6(2):104–113
We set out to develop an alternative simple, accurate and precise method for the determination of artesunate and dihydroartemisinin in bulk samples and dosage forms. The method involves the reaction of the reactive methylene centres generated in situ from the acid decomposition of the artemisinin derivatives with p-dimethylaminobenzaldehyde (DMAB). DMAB was reduced to the purple-coloured alcohol and this was quantitatively used to estimate the concentrations of the artemisinin derivatives. The new procedure was carried out at 60oC in less than 30 minutes with excellent calibration data. The method was accurate and precise and was found equivalent to the International Pharmacopoeia’s method for the assay of these drugs. The method could find application as an alternative in-process and after manufacture method for the quality control of art sunate and dihydroartemisinin.
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