Determination of Total Antioxidant Capacity of Human Plasma from Patients with Lung Diseases Using Constant-Current Coulometry
 
More details
Hide details
1
Department of Analytical Chemistry, А.М. Butlerov Chemical Institute of Kazan State University, Kremlyevskaya, 18, Kazan, Russia, 420008
2
Department of Faculty Therapy, Kazan State Medical University, Butlerova, 40, Kazan, Russia, 420012
CORRESPONDING AUTHOR
Guzel K. Ziyatdinova   

Department of Analytical Chemistry, А.М. Butlerov Chemical Institute of Kazan State University, Kremlyevskaya, 18, Kazan, Russia, 420008 Phone: +7‐843‐2315491 Fax: +7‐843‐2315416
Online publish date: 2006-06-01
Publish date: 2006-06-01
 
Eurasian J Anal Chem 2006;1(1):19–30
KEYWORDS
ABSTRACT
Original coulometric method for total antioxidant capacity (TAC) determination based on the reaction of plasma antioxidants with electrogenerated bromine is developed. TAC is expressed in units of quantity of electricity (Coulombs) spent for titration per a liter of plasma. Coulomb is the universal unit of the TAC measurements because one can express TAC in units of different antioxidants using Faraday constant and the number of electrons involved in the reaction between antioxidant and electrogenerated bromine.The TAC in the plasma being determined for patients with different types of lung diseases is significantly lower than the one for control group. The data obtained by coulometry correlate with results of voltammetric determination based on oxidation of plasma on the glassy carbon electrode (Y=a + bx, where a= -6,7±0,5, b=1,07±0,04, R =0,9934). So, the proposed method could be recommended for clinical laboratories for estimation of antioxidant status and its subsequent correction using additional antioxidant therapy.
 
REFERENCES (36)
1.
Halliwell B, Gutteridge J M C (2000) Free radical in biology and medicine, Clarendon Press: Oxford, p. 160.
 
2.
Menzal D B (1994) The toxicity of air pollution in experimental animals and humans: the role of oxidative stress. Toxicol Lett 72: 267.
 
3.
Podda M, Traber M G, Weber C, Yan L J and Packer L (1998) UV-irradiation depletes antioxidants and causes damage in a model of human skin. Free Rad Biol Med 24: 55.
 
4.
Granot E and Kohen R (2004) Oxidative stress in childhood – in health and disease states. Clin Nutr 23: 3.
 
5.
Deliconstantinos G, Villicotov V and Stavrides J C (1996) Alteration of nitric oxide synthase and xanthine oxidase activates of human keratinocytes by ultraviolet B radiation. Potential role for peroxynitrite in skin inflammation. Biochem Pharmacol 51: 1727.
 
6.
Ginsburg I and Kohen R (1995) Cell damage in inflammatory and infectious sites might involve a coordinated “cross-talk” among oxidants, microbal haemolysins and ampiphiles, cationic proteins, phospholipases, fatty acids, proteinases and cytokines (an overview). Free Radic Res 22: 489.
 
7.
Kohen R (1999) Skin antioxidants: their role in aging and in oxidative stress – new approaches for their evaluation. Biomed Pharmaceother 53: 181.
 
8.
Sies H (1997) Oxidative stress: oxidant and antioxidant. Exp Phisiol 82: 291.
 
9.
Rice-Evance C and Miller N J (1994) Total antioxidant status in plasma and body fluids. Method Enzymol 234: 279.
 
10.
Fang Y Z, Yang S and Wu G (2002) Free radicals, antioxidants, and nutrition. Nutrition 18: 872.
 
11.
Fang Y Z (2002) Theory and Application of Free Radical Biology, Scientific Press: Beijing, p.647.
 
12.
Svistunenko D A, Davies N A, Wilson M T, Stidwill R P and Singer M (1997) Free radical in blood: a measure of haemoglobin autoxidation in vivo. J Chem Soc Perkin Trans 212: 2539.
 
13.
Jackson M J (1999) An overview of methods for assessment of free radical activity in biology. Proc Nutr Soc 58: 1001.
 
14.
Chevion S, Roberts M A and Chevion M (2000) The use of cyclic voltammetry for the evaluation of antioxidant capacity. Free Radic Biol Med 28: 860.
 
15.
Kohen R, Vellaichamy E, Hrbac J, Gati I and Tirosh O (2000) Quantification of the overall reactive oxygen species scavenging capacity of biological fluids and tissues. Free Radic Biol Med 28: 871.
 
16.
Ziyatdinova G K, Budnikov H C and Pogorel’tzev V I (2005) Electrochemical determination of total antioxidant capacity of human plasma. Anal Bioanal Chem 381: 1546.
 
17.
Psotová J, Zahálková J, Hrbáč J, Šimánek V and Bartek J (2001) Determination of total antioxidant capacity in plasma by cyclic voltammetry: Two case reports. Biomed Pap. 145: 81.
 
18.
Campanella L, Bonanni A, Finotti E and Tomassetti M (2004) Biosensors for determination of total and natural antioxidant capacity of red and white wines: comparison with other spectrophotometric and fluorimetric methods. Biosens Bioelectron 19: 641.
 
19.
Pogorel’tzev V I, Ziyatdinova G K and Budnikov H C, Determination of total antioxidant capacity of biological fluids. Patent of Russian Federation 2002134634 from 27.05.05.
 
20.
Van der Vliet A and Cross C E (2000) Oxidants, nitosants and the lung. Am J Med 109: 1.
 
21.
Rahman I and MacNee W (1999) Lung glutathione and oxidative stress: implications in cigarette smoke-induced airway disease. Am J Phys 277: 1067.
 
22.
MacNee W and Donaldson K (1999) Particulate air pollution: injurious and protective mechanisms in the lungs, in: Holgate S T, Samet J M, Koren H S and Maynard R L (Eds.), Air Pollution and Health, Academic Press: San Diego, p.693.
 
23.
Pollack M and Leeuwenburgh C (1999) Molecular mechanisms of oxidative stress in aging: free radicals, aging, antioxidants and disease, in: Sen C K, Packer L and Hänninen O (Eds.), Handbook of Oxidants and Antioxidants in exercise, Elsevier Science: Amsterdam, p.887.
 
24.
Hurst J K, Barrette Jr. W C, Vihinen M and Mantsala P (1989) Leukocytic oxygen activation and microbicidal oxidative toxins. Crit Rev Biochem Molec Biol 24: 270.
 
25.
Klebanoff S J (1980) Oxygen metabolism and the toxic properties of phagocytes. Ann Int Med 93: 480.
 
26.
Hazen S L, Hsu F F, Mueller D M, Crowley J R and Heinecke J W (1996) Human neutrophils employ chlorine gas as an oxidant during phagocytosis. J Clin Invest 98: 1283.
 
27.
Calverley P M A and Walker P (2003) Chronic obstructive pulmonary disease. Lancet 362: 1053.
 
28.
Vernooy J H, Kucukaycan M, Jacobs J A, Chavannes N H, Buurman W A, Dentener M A and Wouters E F (2002) Local and systemic inflammation in patients with chronic obstructive pulmonary disease: soluble tumor necrosis factor receptors are increased in sputum. Am J Respir Crit Care Med 166: 1218.
 
29.
Repine J E, Bast A and Lankhorst I (1997) Oxidative stress in chronic obstructive pulmonary disease. Oxidative stress study group. Am J Respir Crit Care Med 156: 341.
 
30.
Pesci A, Majori M, Cuomo A, Borciani N, Bertacco S, Cacciani G and Gabrielli M (1998) Neutrophils infiltrating bronchial epithelium in chronic obstructive pulmonary disease. Respir Med 92: 863.
 
31.
McCain R W, Holden E P, Blackwell T R and Christman J W (1994) Leukotriene B4 stimulates human polymorphonuclear leukocytes to synthesize and release interleukin-8 in vitro. Am J Respir Cell Mol Biol 10: 651.
 
32.
Zafarullah M, Li W Q, Sylvester J and Ahmad M (2003) Molecular mechanisms of Nacetylcysteine actions. Cell Mol Life Sci 60: 6.
 
33.
Adler V, Yin Z, Tew K D and Ronai Z (1999) Role of redox potential and reactive oxygen species in stress signaling. Oncogene 18: 6104.
 
34.
Lander H M (1997) An essential role for free radicals and derived species in signal transduction. FASEB J 11: 118.
 
35.
Sadowskaa A M, Van Overveld F J, Go´recka D, Zdral A, Filewska M, Demkow U A, Luyten C, Saenen E, Zielinski J and De Backer W A (2005) The interrelationship between markers of inflammation and oxidative stress in chronic obstructive pulmonary disease: modulation by inhaled steroids and antioxidant. Respir Med 99: 241.
 
36.
Gosker H R, Bast A, Haenen G R M M, Fischer M A J G, Van der Vusse G J, Wouters E F M and Schols A M W J (2005) Altered antioxidant status in peripheral skeletal muscle of patients with COPD. Respir Med 99: 118.
 
eISSN:1306-3057