Development and Validation of Ninhydrin Based Colorimetric Spectrophotometric Assay for Determination of Pregabalin in Different Dissolution Mediums
Cheenu Gupta 1
,  
Richa Puri 1
,  
Afzal Hussain 1
,  
Subheet Jain 1  
 
 
More details
Hide details
1
Department of Pharmaceutical Sciences & Drug Research, Punjabi University, Patiala, India
Publish date: 2017-10-25
 
Eurasian J Anal Chem 2013;8(2):90–98
KEYWORDS:
ABSTRACT:
A simple, accurate, cheap and quick spectrophotometric method was developed for the estimation of pregabalin in pharmaceutical pure and dosage forms. Three different mediums (Distilled water, 0.1 N HCl with pH 1.2, buffer with pH 6.8) were taken. The method was developed based on the reaction of ninhydrin with the primary amine present in pregabalin. In all the three cases, reaction produces Rheumann’s Purple colour and absorbs at 571, 555 and 569 nm in distilled water, 0.1 N HCl and pH 6.8 respectively. Beer’s law was followed in the concentration range of 100-200 μg mL-1 in phosphate buffer with pH 6.8, 50-100 μg mL-1 in pH 1.2 and 10-100 μg mL-1 in distilled water. The method developed was successfully applied to pharmaceutical dosage forms with good efficacy and recovery was found from 98.41-99.53%. The method seems to be simple, less time consuming, cost effective and accurate for estimation of pregabalin in pharmaceutical pure and dosage forms.
CORRESPONDING AUTHOR:
Subheet Jain   
Department of Pharmaceutical Sciences & Drug Research, Punjabi University, Patiala, India
 
REFERENCES (8):
1. Bockbrader HN, Radulovic LL, Posvar EL, Strand JC, Alvey CW, Busch JA, Randinitis EJ, Corrigan BW, Haig GM, Boyd RA and Wesche DL (2010) Clinical Pharmacokinetics of Pregabalin in Healthy Volunteers. J Clin Pharmacol 50 : 941-950.
2. Vaidya VV, Yetal MS, Roy MNS, Gomes AN and Joshi SS (2007) LC-MS–MS Determination of Pregabalin in Human Plasma. Chromatographia 66: 925-928. doi: 10.1365/s10337-007-0430-40009-5893/07/12.
3. David B and Christopher M (2005) Analysis of Pregabalin at Therapeutic Concentrations in Human Plasma/Serum by Reversed-Phase HPLC. Therapeutic Drug Monitoring 27[4]: 451-456. doi: 10.1097/01.ftd.0000158874.54100.1a.
4. Gujral RS, Haque SM and Shanker P (2009) Development and validation of pregabalin in bulk, pharmaceutical formulations and in human urine samples by UV spectrophotometry. Int J Biomedical Science 5(2): 175-180.
5. Friedman M (2004) Applications of Ninhydrin Reactions for Analysis of Amino acids, Peptides and Proteins to Agricultural and Biomedical Sciences. J Agric Food Chem 52: 385-389. doi:10.1021/jf030490p.
6. Yonejawa H, Hasuike M and Tatumoto M (1988) Reaction of Ninhydrin with Peptides. Rep Fac Sci Kagoshima Univ 21: 97-104.
7. Harding JV and Warneford HSF. (1916) The Ninhydrin Reaction with Amino-Acids and Ammonium Salts. The J Biological Chemistry 322-327.
8. Lamothe JP and McCormick GP (1972) Influence of acidity on the reaction of ninhydrin with amino acids. Analytical Chemistry 44[4]: 822.
eISSN:1306-3057