Development and Validation of Ninhydrin Based Colorimetric Spectrophotometric Assay for Determination of Pregabalin in Different Dissolution Mediums
Cheenu Gupta 1,   Richa Puri 1,   Afzal Hussain 1,   Subheet Jain 1  
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Department of Pharmaceutical Sciences & Drug Research, Punjabi University, Patiala, India
Subheet Jain   

Department of Pharmaceutical Sciences & Drug Research, Punjabi University, Patiala, India
Publication date: 2017-10-25
Eurasian J Anal Chem 2013;8(2):90–98
A simple, accurate, cheap and quick spectrophotometric method was developed for the estimation of pregabalin in pharmaceutical pure and dosage forms. Three different mediums (Distilled water, 0.1 N HCl with pH 1.2, buffer with pH 6.8) were taken. The method was developed based on the reaction of ninhydrin with the primary amine present in pregabalin. In all the three cases, reaction produces Rheumann’s Purple colour and absorbs at 571, 555 and 569 nm in distilled water, 0.1 N HCl and pH 6.8 respectively. Beer’s law was followed in the concentration range of 100-200 μg mL-1 in phosphate buffer with pH 6.8, 50-100 μg mL-1 in pH 1.2 and 10-100 μg mL-1 in distilled water. The method developed was successfully applied to pharmaceutical dosage forms with good efficacy and recovery was found from 98.41-99.53%. The method seems to be simple, less time consuming, cost effective and accurate for estimation of pregabalin in pharmaceutical pure and dosage forms.
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