Development and Validation of RP-HPLC Method for the Simultaneous Determination of Amiloride Hydrochloride and Furosemide in Pure and Pharmaceutical Dosage Form
More details
Hide details
Sardar Patel College of Pharmacy, INDIA
Jinal Natvarlal Tandel   

Department of Quality Assurance, Sardar Patel College of Pharmacy, Bakrol, Anand, Gujarat, India
Online publication date: 2017-01-04
Publication date: 2017-01-04
Eurasian J Anal Chem 2017;12(4):385–394
A simple, selective, rapid, precise and economical reverse phase high performance liquid chromatographic method has been developed for the simultaneous estimation of Amiloride Hydrochloride and Furosemide from pharmaceutical formulation. The method was carried out on an Enable C18 (250x4.6) mm; 5 µm with a mobile phase consisting of acetonitrile: Water (adjusted to pH 4 using orthophosphoric acid) in the ratio of 70:30 v/v at a flow rate of 1.0 ml/min. Detection was carried out at 281nm. The retention times of Amiloride Hydrochloride and Furosemide were 2.21 min. and 7.60 min., respectively. The developed method was validated according to ICH guidelines for evaluation of accuracy, precision, linearity, limit of detection, limit of quantitation and robustness. The proposed method can be used for the estimation of these drugs in combined dosage form.
Goodman & Gilman’s. (2003). The pharmacological basis of therapeutics. NK: Mcgraw-Hill Medical, pp. 1465-1489.
Tripathi, K. D. (2008). Essential of Medical Pharmacology. New Delhi, India: Jaypee brothers medical publishers (p) Ltd.
Indian Pharmacopoeia. (2010). The Indian Pharmacopoeia Commission. Ghaziabad: Govt. of India Ministry of Health and Family Welfare, (II), pp. 795, 796, 1391-93.
United States Pharmacopoeia & National Formulary. (2007). The United states pharmacopoeia convention Inc., USA. pp. 1373, 2197.
Mohamed, H., Abdel, H., Gazy, A. A., Hassan, E. M., & Belal TS (2008) Derivative and Derivative Ratio Spectrophotometric Analysis of Antihypertensive Ternary Mixture of Amiloride Hydrochloride, Hydrochlorothiazide and Timolol Maleate. Journal of the Chinese Chemical Society, 55(5), 971-978.
IaraLucia, T. D., Jorge, L. S., & Martins, G. N. (2005). Furosemide Determination by First‐Derivative Spectrophotometric Method. Journal of Pharmaceutical Science, 38(7), 1159-1166.
Alaa, E., Emara, S., & Mostafa, A. (2005). HPLC and chemometric-assisted spectrophotometric methods for simultaneous determination of atenolol, amiloride hydrochloride and chlorthalidone. Framac, 60(3), 269–278.
Mostana, S. M., & Elagawish, M. S. (2009). HPLC method for simultaneous determination of amiloride hydrochloride and hydrochlorothiazide in human plasma. Egyptian Journal of Pharmaceutical Sciences, 50, 47-158.
Ibrahima, Y., & Bi-Botti, C. Y. (2013). Validated Reverse Phase High-Performance Liquid Chromatography for Quantification of Furosemide in Tablets and Nanoparticles. Journal of Analytical Methods in Chemistry, 1-9.
Hisham, S., Abou, A., & Mohammad, J. (1998). High-performance liquid chromatographic determination of furosemide in plasma and urine and its use in bioavailability studies. Journal of Chromatography B, 710, 121-128.
Sindhu, J. S., & Charles, B. G. (1993). Simple microscale high- performance liquid chromatographic method for determination of furosemide in neonatal plasma. Journal of Chromatography B. BioMedical Science App., 612(1), 161-165.
Youssef, R. M., Maher, H. M., El-Kimary, El., Hassan, E. M., & Barary, M. H. (2013). Validated Stability-Indicating Methods for the Simultaneous Determination of Amiloride Hydrochloride, Atenolol, and Chlorthalidone Using HPTLC and HPLC with Photodiode Array Detector. Journal of Association of Official Analytical Chemist International, 96(11), 313-323.
Nagori, B. P., & Renu, S. (2010). RP-HPLC Method for Simultaneous Estimation of Frusemide and Amiloride Hydrochloride in Tablet Formulation. Indian Journal of Pharmaceutical Science, 72(3), 384-387.
ICH, Q2 (R1) Validation of Analytical Procedures. (1996). Methodology, International Conference on Harmonization. IFPMA. Geneva.