EVALUATION OF POLYPHENOLIC PROFILE AND
ANTIOXIDANT ACTIVITY OF SOME SPECIES
CULTIVATED IN THE REPUBLIC OF MOLDOVA
1 | Nicolae Testemitanu State University of Medicine and Pharmacy |
2 | State University of Medicine and
Pharmacy NicolaeTestemitanu |
Online publication date: 2018-05-17
Publication date: 2018-05-17
Eurasian J Anal Chem 2018;13(3):emEJAC04023
KEYWORDS
ABSTRACT
The aim of this study was to assess the polyphenolic composition and antioxidant
capacity of the medicinal plants from the collection of the Scientific Center of Medicinal
Plants Cultivation of NicolaeTestemitanu State University of Medicine and Pharmacy:
agrimony - Agrimoniaeupatoria L. (Rosaceae), chicory - Cichoriumintybus L. (Asteraceae.),
artichoke - Cynarascolymus L. (Asteraceae) and St John's-wort - Hypericumperforatum L.
(Hypericaceae). A qualitative and quantitative characterization of the main phenolic
compounds from ethanolic extracts was carried out by HPLC method. The total polyphenolic
and flavonoid contents were measured by spectrophotometric methods. The in vitro
antioxidant activity of these extracts was evaluated using 2,2-diphenyl-1-picrylhydrazyl
(DPPH), Trolox (6-hydroxy-2,5,7,8- tetramethylchroman-2-carboxylic acid) equivalent
antioxidant capacity (TEAC), 2,2′-azinobis (3-ethylbenzthiazoline-6-sulphonic acid (ABTS)
radical cation scavenging and metal chelating activity. Considering the obtained results, the
following order in antioxidant activities was established: A. eupatoria> C. inthybus> H.
perforatum> C. scolymus. All species contain significant amounts of polyphenolic compounds
and they may be considered an important source of natural antioxidants for pharmaceutical
uses.
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