RESEARCH PAPER
Effect of the Interaction of Graphene Oxide Nanoparticles on a Biological Model Cell Membrane
 
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1
Research Centre for Sustainable Process Technology (CESPRO), Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, MALAYSIA
2
Chemical Engineering Programme, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, MALAYSIA
Online publication date: 2018-09-30
Publication date: 2018-09-30
 
Eurasian J Anal Chem 2018;13(5):em60
KEYWORDS
ABSTRACT
Understanding the interaction of graphene oxide (GO) with a lipid membrane is important for the development of tissue engineering and to advance graphene-based biology. In an effort to understand the GO-lipid membrane interaction, appropriate characterisation of GO structure was determined by using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR) and a field emission scanning electron microscope (FESEM). In this study, the lipids 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) were used to produce a lipid vesicle with a conventional gentle hydration method and observed under transmission electron microscopy (TEM). Lipid vesicle-GO interactions were also investigated using dynamic light scattering (DLS) (Malvern Zeta) and TEM by focusing on the effect of the surface charge interactions and localisation of GO on the surface of the vesicle membrane. It was observed that the surface charge of the vesicles increased as the GO nanoparticle concentration increased, but for the low saturation lipid the surface charge remained high as the nanoparticle concentration increased. The localisation and positioning of the GO nanoparticles in the lipid vesicles were confirmed with TEM analysis.
 
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