Effect of the Interaction of Graphene Oxide Nanoparticles on a Biological Model Cell Membrane
More details
Hide details
Research Centre for Sustainable Process Technology (CESPRO), Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, MALAYSIA
Chemical Engineering Programme, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, MALAYSIA
Online publication date: 2018-09-30
Publication date: 2018-09-30
Eurasian J Anal Chem 2018;13(5):em60
Understanding the interaction of graphene oxide (GO) with a lipid membrane is important for the development of tissue engineering and to advance graphene-based biology. In an effort to understand the GO-lipid membrane interaction, appropriate characterisation of GO structure was determined by using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR) and a field emission scanning electron microscope (FESEM). In this study, the lipids 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) were used to produce a lipid vesicle with a conventional gentle hydration method and observed under transmission electron microscopy (TEM). Lipid vesicle-GO interactions were also investigated using dynamic light scattering (DLS) (Malvern Zeta) and TEM by focusing on the effect of the surface charge interactions and localisation of GO on the surface of the vesicle membrane. It was observed that the surface charge of the vesicles increased as the GO nanoparticle concentration increased, but for the low saturation lipid the surface charge remained high as the nanoparticle concentration increased. The localisation and positioning of the GO nanoparticles in the lipid vesicles were confirmed with TEM analysis.
Nel AE, Mädler L, Velegol D, Xia T, Hoek EMV, Somasundaran P, Klaessig F, et al. Understanding biophysicochemical interactions at the nano-bio interface. Nature materials. 2009;8(7):543–557.
Song Z, Wang Y, Xu Z. Mechanical responses of the bio-nano interface: A molecular dynamics study of graphene-coated lipid membrane. Theoretical and Applied Mechanics Letters, (Md). 2015.
Tsuzuki K, Okamoto Y, Iwasa S, Ishikawa R, Sandhu A, Tero R. Reduced graphene oxide as the support for lipid bilayer membrane. Journal of Physics: Conference Series. 2012;352:012016.
Dowhan W, Bogdanov M, Mileykovskaya E, Diacylglycerol DAG. Functional roles of lipids in membranes. Biochemistry of Lipids, Lipoproteins and Membranes, hlm. Sixth Edit. Elsevier. 2016.
Liu X, Chen KL. Interactions of graphene oxide with model cell membranes: Probing nanoparticle attachment and lipid bilayer disruption. Langmuir. 2015;31(44):12076–12086. acs.langmuir.5b02414.
Rao CNR, Maitra U, Matte HSSR. Synthesis, characterization, and selected properties of graphene. 2013.
Novoselov KS, Fal′ko VI, Colombo L, Gellert PR, Schwab MG, Kim K. A roadmap for graphene. Nature. 2012;490(7419):192–200.
Perreault F, Fonseca de Faria A, Elimelech M. Environmental applications of graphene-based nanomaterials. Chem. Soc. Rev. 2015;44(16):5861–5896.
Titov aV, Král P, Pearson R. Sandwiched graphene-membrane superstructures. ACS nano. 2009;4(1):229–234.
Hirtz M, Oikonomou A, Georgiou T, Fuchs H, Vijayaraghavan A. Multiplexed biomimetic lipid membranes on graphene by dip-pen nanolithography. Nature communications. 2013;4(May):2591.
Sanchez VC, Jachak A, Hurt RH, Kane AB. Biological interactions of graphene-family nanomaterials: An interdisciplinary review. Chem. Res. Toxicol. 2012;25(1):15–34.
Frost R, Svdhem S, Langhammer C, Kasemo B. Graphene oxide and lipid membrane: size-independent interaction. Langmuir. 2016.
Wu L, Zeng L, Jiang X. Revealing the nature of interaction between graphene oxide and lipid membrane by surface-enhanced infrared absorption spectroscopy. Journal of the American Chemical Society. 2015;137(32):10052–10055.
Frost R, Jönsson GE, Chakarov D, Svedhem S, Kasemo B. Graphene oxide and lipid membranes: Interactions and nanocomposite structures. Nano Letters. 2012;12(7):3356–3362.
Longfei R, Jiaojiao L, Jingliang L, Yuyan W, Yujiang D, Bing Y, Kai Y, Yuqiang M. Reduced graphene oxide directed self-assembly of phospholipid monolayers in liquid and gel phases. Biochim Biophys acta. 2015;1848(2015):1203-1211.
Munawar H, Mohd K, Rahman IA, Ahmad AF, Radiman S, Mohamed F, Yasir MS. Effect of titanium dioxide nanoparticle addition to the surface charge and structure of DPPC vesicles (Kesan Penambahan Nanozarah Titanium Oksida ke atas Cas Permukaan dan Struktur Vesikel). Malaysian Journal of Analytical Sciences. 2015;19(1):179–183.
Matter S, Nordin D, Yarkoni O, Savinykh N, Frankel D. Revealing the selective interactions of fibronectin with lipid bilayers, Soft Matter. 2011;7(22):10666–10675.
Shahriary L, Athawale Aa. Graphene oxide synthesized by using modified Hummers approach. International Journal of Renewable Energy and Environmental Engineering. 2014;02(01):58–63.
Challan S, Massound A. Radiolabeling by graphene oxide by tchnetrium-99m for infection imaging in rats. J. Radioanal Nucl Chem. 2017;314:2189–2199.
Leffler J. Towards graphene based transparent conductive coating 86 (Master’s thesis). 2012.
Baskoro F, Wong C, Kumar SR, Chang C, Chen C. Graphene oxide-cation interaction : Inter-layer spacing and zeta potential changes in response to various salt solutions. Journal of Membrane Science. 2018;554:253–263.
Liu D, Huang G, Yu Y, He Y, Zhang H, Cui H. N-(Aminobutyl)-N- (ethylisoluminol) and hemin dual-functionalized graphene hybrids with high chemiluminescence. Chemical Communications. 2013;49(84):9794.
Javed SI, Hussain Z. Covalently functionalized graphene oxide – characterization and its electrochemical performance. Int. J. Electrochem. Sci. 2015;10:9475–9487.
Bykkam S, Rao K. Synthesis and characterization of graphene oxide and its antimicrobial activity against Klebsiella and Staphylococus. International Journal of Advanced Biotechnology and Research. 2013;4(1):142–146. Retrieved from
Fahrul M, Hanifah R, Jaafar J, Aziz M, Fauzi A, Rahman MA, Dzarfan MH. Synthesis of graphene oxide nanosheets via modified Hummers ‘ method and its physicochemical properties. Jurnal Teknologi. 2015;1:189–192.
Liu Z, Duan X, Zhou X, Qian G, Zhou J, Yuan W. Controlling and formation mechanism of oxygen-containing groups on graphite oxide. Industrial and Engineering Chemistry Research. 2014;53(1):253–258.
Li S, Stein AJ, Kruger A, Leblanc RM. Head groups of lipids govern the interaction and orientation between graphene oxide and lipids. Journal of Physical Chemistry. 2013;117(31):16150-16158.
Koopal L, Hlady V, Lyklema J. Electrophoretic study of polymer adsorption: dectran, polyethylene oxide and polyvinyl alcohol on silver iodide. J. Colloid Interface Sci. 1988;121:49–62.
Somerharju P, Virtanen JA, Cheng KH. Lateral organization of membrane lipids – The superlattice view. Biochim Biophys Acta mol Cell Biol Lipids. 1999;1440:32–48.