High Performance Liquid Chromatographic Methods for Analysis of Taurine in Energy Drinks after Pre-column Derivatization
Maida Omer 1,   Mei Omar 2,   Andreas Thiel 3,   Abdalla Elbashir 4  
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Department of Chemistry, Faculty of Science, Sudan University of Science and Technology, Khartoum, SUDAN
Central Laboratory, Ministry of Higher Education & Scientific Research, P. O. Box Office 7099, Khartoum, SUDAN
Universität Kassel, Ökologische Agrarwissenschaften, Steinstr. 19, 37213 Witzenhausen, GERMANY
Department of Chemistry, Faculty of Science, University of Khartoum, Khartoum, SUDAN
Publication date: 2018-07-14
Eurasian J Anal Chem 2018;13(5):em40
Simple and efficient high performance liquid chromatography (HPLC) with photodiode array (PDA) and fluorescence (FLD) detection methods have been validated for determination of taurine in energy drinks. These methods are based on pre-column derivatization of taurine with 4-chloro-7-nitrobenzo-2-oxa-1,3- diazole (NBD-Cl) at alkaline medium to form colored fluorescent product. In the both validated HPLC methods, the derivatization product is separated on Intersil ODS-3 analytical column with acetonitrile and 0.1% trichloroacetic acid (30:70, v:v) as mobile phase. The eluted derivative is detected at 472 nm for HPLC-PDA and 472 nm/530 nm (Ex/Em) for HPLC-FLD. The methods were validated in terms of linearity, limit of detection (LOD), limit of quantification (LOQ), precision and accuracy (recovery). Good linearities were achieved for taurine (r2> 0.9998 and 0.9993) in the concentration range of 5-50 mg L-1 and 5-50 µg L-1 for HPLC-PDA and HPLC-FLD respectively. The LODs were 0.296 and 0.616 × 10-3 mg L-1 for HPLC-PDA and HPLC-FLD respectively. The precision for peak area were 0.78 and 0.61% for HPLC-PDA and HPLC-FLD respectively. Recoveries of taurine ranging from 92-103.3%), (n = 3) were obtained. The validated method was successfully applied for the determination of taurine in some selected energy drinks available in local markets.
Rai KP, Rai HB, Dahal S, Chaudhary S, Shrestha S. Determination of Caffeine and Taurine Contents in Energy Drinks by HPLC-UV. Journal of Food Science and Technology Nepal. 2016;9:66-73.
Gliszczyńska-Świgło A, Rybicka I. Simultaneous determination of caffeine and water-soluble vitamins in energy drinks by HPLC with photodiode array and fluorescence detection. Food Analytical Methods. 2015;8(1):139-146.
Tsvetkova DD, Klisurov RC, Pankova SA, Zlatkov BA. Investigation of some pharmacological effects of Caffeine and Taurine in food supplements. Int. J. Nutr. Food Sci. 2015;4(1):18-23.
Schaffer SW, Jong CJ, Ramila K, Azuma J. Physiological roles of taurine in heart and muscle. Journal of Biomedical Science. 2010;17(1):S2.
Triebel S, Sproll C, Reusch H, Godelmann R, Lachenmeier DW. Rapid analysis of taurine in energy drinks using amino acid analyzer and Fourier transform infrared (FTIR) spectroscopy as basis for toxicological evaluation. Amino Acids. 2007;33(3):451-457.
Todorova KA, Pencheva IP. Analytical approaches and methods in quality control procedures of energy food drinks containing caffeine and taurine. International Journal of Nutrition and Food Sciences. 2015;4(1-1):1-8.
Sawabe Y, Tagami T, Yamasaki K. Determination of taurine in energy drinks by HPLC using a pre-column derivative. Journal of health science. 2008;54(6):661-664.
Nejdl L, Sochor J, Zitka O, Cernei N, Ruttkay-Nedecky B, Kopel P, Babula P, Adam V, Hubalek J, Kizek R. Spectrometric and chromatographic study of reactive oxidants hypochlorous and hypobromous acids and their interactions with taurine. Chromatographia. 2013;76(7-8):363-373.
McConn B. Determination of taurine in energy drinks by high-performance liquid chromatography. Concordia Coll J Anal Chem. 2012;3:47-52.
Draganov GB, Pencheva IP, Todorova KA. UV-spectrophotometry determination of taurine in energy drink mixtures. Int. J. Nutr. Food Sci. 2014;3(2):123-126.
Chen Z, Chen B, Yao S. High-performance liquid chromatography/electrospray ionization-mass spectrometry for simultaneous determination of taurine and 10 water-soluble vitamins in multivitamin tablets. Analytica Chimica Acta. 2006;569(1):169-175.
Hyánová B, Opekar F, Tůma P. Simultaneous and rapid determination of caffeine and taurine in energy drinks by MEKC in a short capillary with dual contactless conductivity/photometry detection. Electrophoresis. 2014;35:1660-1665.
Hohmann M, Felbinger C, Christoph N, Wachter H, Wiest J, Holzgrabe U. Quantification of taurine in energy drinks using 1H NMR, J. Pharm. Biomed. Anal. 2014;93:156-160.
Mohamed MKS, A Elbashir A, Y Aboul-Enein H. Spectrophotometric Method for Determination of Taurine in Energy Drinks. Current Pharmaceutical Analysis. 2017;13(1):11-17.
Sharaf El Din M, Wahba M. Novel validated spectrofluorimetric methods for the determination of taurine in energy drinks and human urine. Luminescence. 2015;30(2):137-143.
Ziegler F. Le Boucher J, Coudray-Lucas C, Cynober L. Plasma amino-acid determinations by reversed-phase HPLC: Improvement of the orthophthalaldehyde method and comparison with ion exchange chromatography. Journal of Automatic Chemistry. 1992;14(4):145-149.
Inoue H, Fukunaga K, Tsuruta Y. Determination of taurine in plasma by high-performance liquid chromatography using 4-(5, 6-dimethoxy-2-phthalimidinyl)-2-methoxyphenylsulfonyl chloride as a fluorescent labeling reagent. Analytical biochemistry. 2003;319(1):138-142.
McMahon GP, O’Kennedy R, Kelly MT. High-performance liquid chromatographic determination of taurine in human plasma using pre-column extraction and derivatization. Journal of pharmaceutical and biomedical analysis. 1996;14(8-10):1287-1294.
Önal A. Spectrophotometric and spectrofluorimetric determination of some drugs containing secondary amino group in bulk drug and dosage forms via derivatization with 7-chloro-4-nitrobenzofurazon. Química Nova. 2011;34(4):677-682.
Omar MMA, Elbashir AA, Schmitz OJ. Capillary electrophoresis method with UV-detection for analysis of free amino acids concentrations in food. Food chemistry. 2017;214:300-307.
Götz S, Revermann T, Karst U. Quantitative on-chip determination of taurine in energy and sports drinks. Lab on a Chip. 2007;7(1):93-97.
Begum A. Development and validation of acetylcysteine and taurine in tablet dosage form by RPHPLC. International Journal of Universal Pharmacy and Bio Sciences. 2014;3(5):11-24.
Chirita R-I, Dascalu C, Gavrila L, Elfakir C. Simultaneous Analysis of Taurine and Caffeine in Energy Drinks using Hydrophilic Interaction Chromatography with UV and Evaporative Light Scattering Detection on line. Revista De Chimie. 2010;61(12):1173-1176.
Ferreira I, Nunes M, Mendes E, Remiao F, Ferreira M. Development of an HPLC-UV method for determination of taurine in infant formulae and breast milk. Journal of liquid chromatography & related technologies. 1997;20(8):1269-1278.