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In vitro α-Glucosidase and α-Amylase Enzyme Inhibitory Effects in Elaeagnus angustifolia Leaves Extracts
| 1 | Anadolu University, TURKEY |
| 2 | Ege University, TURKEY |
| 3 | Mehmet Akif Ersoy University, TURKEY |
CORRESPONDING AUTHOR
Hale Secilmis Canbay
Department of Bioengineering, Faculty of Engineering and Architecture, Mehmet Akif Ersoy University, Burdur, 15030, Turkey
Department of Bioengineering, Faculty of Engineering and Architecture, Mehmet Akif Ersoy University, Burdur, 15030, Turkey
Online publication date: 2016-12-13
Publication date: 2016-12-13
Eurasian J Anal Chem 2017;12(2):117–126
KEYWORDS
TOPICS
ABSTRACT
This study was performed to demonstrate α-glucosidase and α-amylase enzyme inhibitor activities using the methanolic extracts of Elaeagnus angustifolia leaves. Methanolic extracts were prepared by two different extraction methods [accelerated solvent extractor (ASE) and Soxhlet apparatus]. The analytical procedure involved the application of liquid chromatography. Our results showed that the ASE extracts inhibited α-amylase (10-100 µg/ ml) and α-glucosidase (10-50 µg/ml) dose-dependently exhibitedrp inhibitory activities with the extracts [α-amylase (40%) and α-glucosidase (56%)]. RP-HPLC analyses of the methanolic extracts were detected with vanillic acid and 4-hydroxybenzoic acid as the main phenolic acids in all the ASE extracts. Elaeagnus angustifolia leaf extracts may be attributed to the presence of vanillic acid and 4-hydroxybenzoic acid, there by confirming with its traditional use for the management of diabetes mellitus. We implicate that the anti-diabetic activity through in vitro assessments and support to the folkloric use of this plant for controlling type-2 diabetes mellitus in Turkey.
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