Interaction and Fluorescence Quenching Study of Levofloxacin with Divalent Toxic Metal Ions
 
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Department of Chemistry, Aligarh Muslim University, Aligarh-202001, India
CORRESPONDING AUTHOR
Khwaja S. Siddiqi   

Department of Chemistry, Aligarh Muslim University, Aligarh-202001, India
Publish date: 2017-10-30
 
Eurasian J Anal Chem 2010;5(2):177–186
KEYWORDS
ABSTRACT
The binding interaction of levofloxacin (LF) with toxic metal ions Cd+2, Hg+2 and Pb+2 was investigated in aqueous acidic medium by absorption and fluorescence spectrophotometry. The experimental results showed that the metal ions quench the fluorescence intensity of LF by forming LF2 -metal complex. It was found that static quenching was the main reason of fluorescence quenching. Quenching of Lf by toxic metal follows the order Hg>Cd>Pb. The stoichiometry and logK of formed chelate was determined by absorption and fluorescence spectrophotometry. The quenching constant Ksv and the binding sites “n” were determined together with their thermodynamic parameters at 25 ºC and 35 ºC. The positive entropy change indicated the gain in configurational entropy as a result of chelation. The process of interaction was spontaneous and mainly ∆S-driven.
 
REFERENCES (23)
1.
Croisier D, Etienne M, Bergoin E, Charles P-E, Lequeu C, Piroth L, Portier H, and Chavanet P (2004) Mutant selection window in LF and moxifloxacin treatments of experimental pneumococcal pneumonia in a rabbit model of human therapy. Antimicrob Agents Chemother 48:1699.
 
2.
Roblin P M, and Hammerschlag M R (2003) In vitro activity of a new antibiotic NVPPDF386 (VRC4887) against chlamydia pneumoniae. Antimicrob Agents Chemother 47:1447.
 
3.
Owens R C J, and Ambrose P G, (2000) Clinical use of the fluoroquinolones. Med Clin North Am 84:1447.
 
4.
Barton J B, and Michael J P (2008) Recent advances in bacterial topoisomerase inhibitors. Current Opinion in Pharmacology 8:574.
 
5.
Zupancic T and Pihlar B (1999) Preconcentration of quinolones by dialysis on-line coupled to high-performance liquid chromatography. J Chromatogr (A) 840:11.
 
6.
Shtykov S N, Smirnova T D, Bylinkin Y G, Kalashnikova N V, and Zhemerichkin D A (2007) Determination of Ciprofloxacin and Enrofloxacin by the Sensitized Fluorescence of Europium in the Presence of the Second Ligand and Micelles of Anionic Surfactants. J Anal Chem 62:136.
 
7.
Waggoner T B, Bowman, M C, (1987) Spectrofluorometric determination of BAY Vp 2674 residues in poultry tissues. J Assoc Off Anal Chem 70:813.
 
8.
Veiopoulou C J, Ioannou P C and Liaidou E S (1997) Application of terbium sensitized fluorescence for the determination of fluoroquinolone antibiotics pefloxacin, ciprofloxacin and norfloxacin in serum. J Pharm Biomed Anal 15: 1839.
 
9.
Turel I, Golobi P A, Klazar A, Pihlar B, Buglyo P, Tolib E, Rehder D, and Sepiv, K. (2003) Interactions of oxovanadium(IV) and the quinolone family memberciprofloxacin. J Inorg Biochem 95:199.
 
10.
Kilic E, Koseoglu F and Akay M A (1994) The non-aqueous titrimetric assay of selected antibiotics using tetra-N-butylammonium hydroxide as titrant. J Pharm Biomed Anal 12:347.
 
11.
Mostafa S, El-sadek M and Aalla, E A (2002) Spectrophotometric determination of ciprofloxacin, enrofloxacin and pefloxacin through charge transfer complex formation. J Pharm Biomed Anal 27:133.
 
12.
Liu Z, and Huang C R (2000) Study of the fluorescence characteristics of norfloxacin in reversed micelles and application in analysis. Analyst 125:1477.
 
13.
Trindade M A G, Cunha P A C, de-Arauja T A, Dasilva G M and Ferreira V S (2006) Interaction of moxifloxacin with Cu(II) ions using square wave voltametry and its application in the determination in tablets. Ecl Quim Sao Paulo 31:31.
 
14.
Fierens C, Hillaert S and Bossche W V (2000) The qualitative and quantitative determination of quinolones of first and second generation by capillary electrophoresis. J Pharm Biomed Anal 22:763.
 
15.
Novakovic J, Nesmark K, Nova H and Filka K (2001) An HPTLC method for the determination and the purity control of ciprofloxacin HCl in coated tablets. J Pharm Biomed Anal 25:957.
 
16.
Siddiqi K S, Mohd A, Khan A A P, and Bano S, (2009) Interaction of CFP with Metal ions: Complex Formation of CFP with Metal ion by Absorption and Fluorescence Spectrophotometery. J Korean Chem Soc 53:152.
 
17.
Guo M, Zou J W, Yi, PG, Shang Z C, Hu, G X and Yu Q S (2004) Binding interaction of Gatifloxacin with Bovine Serum Albumin. Anal Sci 20;465.
 
18.
Wang C, Wu Q H, Li C R, Wang Z, Ma J J, Zang X H andQin N X, (2007) Interaction of Tetrandrine with Human Serum Albumin: a Fluorescence quenching study. Anal Sci 23:429.
 
19.
Lakowicz J R, Principles of fluorescence spectroscopy. Plenum Press, NewYork. 2nd ed., p. 698 1999.
 
20.
Posokhov Y, Kus M, Biner H, gumus M K, Tugcu F T, Aydemir E, Kaban S and Icli S, (2004) Spectral properties and complex formation with Cu 2+ ions of 2- and 4-(Narylimino) quinolines. J Photochem Photobiol 161:247.
 
21.
Siddiqi K S, Bano S, Mohd A, and Khan A A P, (2009) Binding interaction of captopril with metal ions: A fluorescence quenching study. Chinese J Chem 27:1755.
 
22.
Xu Y, Shen H X, Huan H G (1997) Fluorescence characteristics of iron(III)- norfloxacin-guanylic acid ternary complex. Anal Chem 25:419.
 
23.
Calvin M, and Melchior N C (1948) Stability of chelate compounds IV: Effect of metal ion. J Am Chem Soc 70:3270.
 
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