Isolation, Characterization and Determination of Nordihydroguaiaretic Acid in Nutrition Supplement by Using Reversed Phase-High Performance Liquid Chromatography
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Vignan Pharmacy College Vadlamudi, India
Karthikeyan Ramadoss   

Department of Phytopharmaceutical and Biological analysis, Vignan Pharmacy College, Vadlamudi, Guntur-522 213. Andhra Pradesh, India
Publication date: 2016-07-28
Eurasian J Anal Chem 2016;11(4):225–232
Nordihydroguaiaretic acid (NDGA) is a lignan found in large amounts in the ethnobotanically important plant, Larrea tridentata (Moc. & Sess.) Cov. (Zygophyllaceae). So far NDGA was not isolated and estimated in larrea tridentata (moc. & sess.) cov. In this study isolation of Nordihydroguaiaretic acid was achieved by preparative TLC and the compound thus isolated was characterised by Ultraviolet and Mass spectral analysis. Mass spectral data shows molecular ion peak of m/z=302.2. An isocratic RP-HPLC method was developed for the estimation of Nordihydroguaiaretic acid in marketed formulation. Retention time of Nordihydroguaiaretic acid was found as 4.173 min. This method has obeyed linearity over the concentration range of 10-50µg/ml and the regression coefficient obtained from linearity plot for Nordihydroguaiaretic acid was found as 0.999. RP-HPLC method was validated in pursuance of International conference on Harmonization (ICH) guidelines. This method is simple and economic one and it could be used for the estimatimation and routine analysis of the NDGA in any crude drug extract and in any formulation.
Anjaneyulu M, Chopra K (2004). Nordihydroguairetic acid, a lignin, prevents oxidative stress and the development of diabetic nephropathy in rats. Journal of Pharmacology 72(1), 42–50.
Arteaga S, Andrade-Cetto A (2005). Larrea tridentate (Creosote bush), an abundant plant of Mexican and US-American deserts and its metabolite nordihydroguaiaretic acid. Journal of Ethnopharmacology 98 (3) 231– 239.
Capdevila J, Gil L., Orellana M (1988). Inhibitors of cytochrome P-450 dependent arachidonic acid metabolism. Archives of Biochemistry and Biophysics 261(2), 257–263.
Craigo J, Callahan M, Huang RC C (2000). Inhibition of human papillomavirus type 16 gene expression by nordihydroguaiaretic acid plant lignan derivatives. Antiviral Research 47(1),19–28.
Floriano-S´anchez E, Villanueva C (2006). Nordihydroguaiaretic acid is a potent in vitro scavenger of peroxynitrite, singlet oxygen, hydroxyl radical, superoxide anion and hypochlorous acid and prevents in vivo ozone-induced tyrosinenitrationin lungs. Free Radical Research 40, 523–533.
ICH. 1996. Guideline Q2B Validation of Analytical Procedures” Methodology.
Jang YS, Her SJ, (2003). Nordihydroguaiaretic acid, an antioxidant, inhibits transforming growth factor-beta activity through the inhibition of Smad signaling pathway. Experimental Cell Research 289(2), 335-41.
Julianne M, Alberto Z, Leon A (1986). High Performance Liquid Chromatographic Determination of Nordihydroguaiaretic Acid. Analytical Letters 19, 13-14.
Lambert J D, Zhao D, Meyers RO (2002). Nordihydroguaiaretic acid: hepatotoxicity and detoxification in the mouse. Current pharmaceutical research 40(12), 1701–1708.
Lambert J, Dorr R (2004). Nordihydroguaiaretic Acid: A Review of Its Numerous and Varied Biological Activities. Informa health care 42(2), 149-158.
Li Xiu-Qin, Yang Min- Li, (2009). Analysis of synthetic antioxidants and preservatives in edible vegetable oil by HPLC/TOF-MS. Food Chemistry 113, 692–700.
McDonald RW, Bunjobpon W, (2001). Synthesis and anticancer activity of nordihydroguaiaretic acid (NDGA) and analogues. Translational biomedicine 16(6), 261-70.
Sebastian T, Roberto D (2011). Potential use of low-NDGA Larrea divaricata extracts as antioxidant in foods. Revista peruvia biologica 18(2), 159 - 164.
Sheikh NM, Philen RM (1997). Chaparral- associated hepatotoxicity. Archives of Internal Medicine 157(8), 913–919.
Smith A Y, Feddersen RM (1994). Cystic renal cell carcinoma and acquired renal cystic disease associated with consumption of chaparral tea: a case report. Journal of Urology 152(6),2089–2091.
Vargas-Arispuro R, Reyes B (2005). Antifungal lignans from the creosotebush (Larrea tridentata). Industrial Crops and Products 22(2), 101–107.
Yam-Canul P, Sanchez-Gonz´alez DJ (2008). Nordihydroguaiaretic acid attenuates potassium dichromate- induced oxidative stress and nephrotoxicity. Food and Chemical Toxicology 46(3), 1089–1096.
Z. klin. Chem, u. kiln (1970). Analytical and Preparative Thin-Layer Chromatography of Porphyrin Methyl Esters. Biochem. 8. Jg., S. 197—207.
Sai Koushik O, Srinivasa Babu P, Ramadoss Karthikeyan (2015). Isolation of Anticancer Bioactive and In Vitro Evaluation of Antioxidant and Anticancer Activity of Cynodon dactylon (L). Pers.Translational biomedicine 6(3),23-31.
C. Duisberg, L. B. Shires, and C. W. Botkin (1949). Determination of Nordihydroguaiaretic Acid in Leaf of Larrea divaricata (Creosote Bush). Analytical Chemistry 21 (11), 1393-1396.