Liquid Chromatography with Indirect UV Detection for Determination of Some Inorganic Anions Using a Permanently Coated Column: Determination of Monofluorophosphate, Phosphate and Iodide
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Helwan University, Faculty of Pharmacy, Analytical Chemistry Department, Cairo, Egypt
Mohamed Salem Rizk   

College of Pharmacy Helwan University, Ain Helwan – Cairo (POB 11795), Egypt
Online publication date: 2008-03-01
Publication date: 2008-03-01
Eurasian J Anal Chem 2008;3(2):223–235
A method for the separation and determination of inorganic anions using a reversed phase column permanently coated with n-cetylpyridinium chloride is described. A 1 mM sodium salicylate solution in 5% (v/v) methanol was used as the mobile phase. The separation of some inorganic anions (monofluorophosphate (MFP), phosphate and iodide) was carried out in about 10 minutes. Detection was made using indirect UV detection at 290 nm. Satisfactory reproducibility of the results was obtained even after a four months period of continual use of the column. Through the evaluation of the analytical parameters, the method was linear (r = 0.999) at concentrations ranging from 10.0 to 100.0 μg mL-1.
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