New Extractive Colorimetric Method for the Assay of Gentamicin and Streptomycin Using Ion-Pair Association Complexes
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Department of Chemistry, College of Science, Aljouf University Sakaka-Saudi Arabia
Safwan M. Fraihat   

Department of Chemistry, College of Science, Aljouf University Sakaka-Saudi Arabia
Publish date: 2017-10-05
Eurasian J Anal Chem 2015;10(3):180–186
Gentamicin and streptomycin aminoglycozide antibiotics, were determined using new selective spectrophotometric methods based on the formation of stable ion association complexes with methyl red dye in alkaline medium. These complexes can be extracted with chloroform at (pH = 8.5) after that the absorbance of the colored complexes was measured at 490 nm. The methods were linear in the concentration ranges of 15-60 and 18-75 with Limit of detection (LOD) of 9 and 13 µgmL-1 for gentamicin and streptomycin respectively. The stoichiometry of the two ion pair complexes was determined by the continuous variation method.
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