New RP-HPLC Method for The Determination of Olmesartan Medoxomil in Tablet Dosage Form
More details
Hide details
HITS College of Pharmacy, Bogaram (V), Hyderabad, Andhara Pradesh, India
Analytical R & D, Hetero Drugs LTD, Hyderabad, Andhara Pradesh, India
Publish date: 2017-10-27
Eurasian J Anal Chem 2010;5(2):145–151
A simple rapid, sensitive, accurate, precise and reproducible high performance liquid chromatographic method has been developed to assay Olmesartan medoxomil in tablet dosage form. The HPLC analysis used a reversed phase Hypersil BDS C8 (250X4.6mm, 5µm) column and a mobile phase constituted of buffer and acetonitrile (55:45 % v/v). The buffer is composed of 3gm of sodium perchlorate and 3ml of tri ethyl amine in 1000 mL of water and the pH of the solution was adjusted to 3.0 with orthophosphoric acid. The wave length of the detection is 250 nm. The validation data showed that the assay is sensitive, specific and reproducible for the determination of olmesartan in the dosage form. The method is linear from 10µg mL-1 to 120µg mL-1. The accuracy of the method was found to be 99.54%. Mean inter and intraday assay relative standard deviation (RSD) were less than 1.0%. The proposed method provided an accurate and precise analysis of olmesartan in its pharmaceutical dosage form.
Raveendra B. Ganduri   
HITS College of Pharmacy, Bogaram (V), Hyderabad, Andhara Pradesh, India
1. Norwood D, Branch E, Smith B and Honeywell M (2002) Olmesartan medoxomil for hypertension: a clinical review, Drug forecast. P&T 27 (Suppl 12):611.
2. Chrysant SG and Chrysant GS (2004) Expert Opin Pharmacother 37:657. doi:10.1517/ 14656566.5.3.657.
3. Chrysant SG, Weber MA, Wang AC and Himman DJ (2004) Evaluation of antihypertensive therapy with the combination of olmesartan medoxomil and hydrochlorthiazide. Am J Hypertens 17:252. doi:10.1016/j.amjhyper.2003.11.003.
4. Marshall TG, Lee RE and Marshall FE (2006) Common angiotensin receptor blockers may directly modulate the immune system via VDR,PPAR and CCR2b.Theor Biol Med Model 3:1.doi:10.1186/1742-4682-3-1.
5. Vikas V (2008) LC-MS-MS determination of olmesartan in human plasma. Chromatographia 67(1):1.
6. Chen SH (2008) HPLC-MS for determining olmesartan in human plasma. NanFang Vike Da Xue Xue Bao 28(60):1104.
7. Liud (2007) Quantitative determination of olmesartan in human plasma and urine by LC coupled to tandem mass spectrometry. J Chromatogr B 856(1):190.
8. Liu JF Wang (2006) Determination of olmesartan in human plasma by HPLC with fluorescence detection. J Pharm Anal 26(5):686.
9. Murakami T, Konno H, Fukutsu, Onodera M, Kawasaki T and Kusu F (2008) Identification of a degradation product in stressed tablets of olmesartan medoxomil by the complementary use of HPLC hyphenated techniques. J Pharm Biomed Anal 47(3):553.
10. Vipul P Rane, Kiran R Patil, Jaiprakash N Sangshetti, Ravindra D Yeole and Devanad B Shinde (2009) Stability indicating LC method for the determination of olmesartan in bulk drug and in pharmaceutical dosage form. Chromatographia 69(1):169.
11. Lisiane Bajerski, Rochele C Rossi, Carolina L Dias, Ana M Bergold and Pedro E Froehlich (2008) Stability indicating LC Determination of a new antihypertensive, olmesartan medoxomil in tablets. Chromatographia 68(11):991.
12. Celebier M and Altinoz S (2007) Determination of olmesartan medoxomil in tablets by UV-Vis Spectrophotometry. Pharmazie 62(6):419.
13. Piyush Trivedi, Kartikeyan.C, Raman Kachave and Rajendra Bhadane (2009) stability-indicating assay method for estimation of olmesartan medoxomil and its metabolite. Journal of Liquid Chromatography & Related Technologies 10:1516.
14. Patel CV, Khandhar AP, Captain AD and Patel KT (2007) Validated absorption factor spectrophotometric and Reverse-phase High Performance Liquid Chromatography methods for the determination of Ramipril and Olmesartan Medoxomil in pharmaceutical formulations. Eurasian J Anal Chem 2:159.
15. ICH (1996) Harmonized tripartite guideline: validation of analytical procedures:Methodology.Q2B.
16. ICH (1996) Harmonized tripartite guideline: validation of analytical procedures.Q2A.