New Study of Anion-Exchange Chromatography for Profiling of Soil and Peat Humic Substances and Aromatic Carboxylic Acids
Janka Ráczová 1  
,  
 
 
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Department of Analytical Chemistry, Faculty of Natural Sciences, Comenius University in Bratislava, Mlynská dolina CH-2, 842 15 Bratislava, Slovak Republic
CORRESPONDING AUTHOR
Janka Ráczová   

Department of Analytical Chemistry, Faculty of Natural Sciences, Comenius University in Bratislava, Mlynská dolina CH-2, 842 15 Bratislava, Slovak Republic
Publish date: 2017-10-04
 
Eurasian J Anal Chem 2014;9(1):15–24
KEYWORDS
ABSTRACT
A simple anion-exchange liquid chromatographicmethod has been developed to assay commercial available humic substances, alkaline extracts of various types of soils and selected model aromatic acids. The anion-exchange HPLC analysis used short glass column (30x3 mm) filled with Separon HEMA-BIO 1000 sorbent with (diethylamino)ethyl functional groups (60 μm particles) and a mobile phase A constituted of aqueous solution of tetrasodium salt of EDTA (pH 12.0; 5 mmol L-1) and mobile phase B constituted of aqueous solution of tetrasodium salt of EDTA (pH 12.0, 500 mmol L-1). The wavelength of the detection is 480 nm (lex) and 530 nm (lem) for humic substances and soils and 280 nm for aromatic acids. The proposed method provided an accurate and precise analysis of commercial available humic substances, alkaline extracts of various types of soils and selected aromatic acids. It was experimentally proved that under the selected conditions model aromatic acids are separated according to increasing effective negative charge and by analogy we suppose the same separation model also for fulvic acids and humic acids.
 
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