One and Two‐dimensional Nuclear Magnetic Resonance Spectroscopy Analysis of Enoxaparin Samples
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Publication date: 2019-05-23
Eurasian J Anal Chem 2019;14(2):emEJAC191036
Heparin has been used as a clinical anticoagulant for more than 50 years and it has been the second most used drug in the world. Unfractionated heparin are broke down by either specific lyase enzymes or chemical depolymerizations to obtain various of low molecular weight heparins (LMWHs) maintains much of the antithrombotic activity and provides a more predictable pharmacokinetic profile and a ease of use. Although this process is believed to be well regulated and inspected, LMWHs may still show distinct pharmacological and biochemical profiles primarily because of their structural differences. These differences which derived from LMWHs preparation process can be accurately and precisely identified by nuclear magnetic resonance (NMR) spectroscopy. This study is designed to compare the structural differences of 11 enoxaparin copies from different manufacturers. Using a combination of one‐ and two‐dimensional high sensitivity NMR, we were able to accurately integrate the monosaccharide residues of LMWHs and exhibit the structural differences.