Phenyl butyrate acid inhibit tnf-alpha-induced nuclear atf6 expression in endothelial cells
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Online publish date: 2019-05-25
Publish date: 2019-05-25
Eurasian J Anal Chem 2019;14(2):emEJAC190299
Endothelial cell (EC) is important tissue that has high plasticity in response to vascular millieu change. However, its plasticity turn EC lead to its dysfunction and contribute in several disease patomechanism. An inflammation agent such as TNF-α can induce EC dysfunction via endoplasmic reticulum stress (ERS) pathway. Targeting ERS was new approach in vascular biology to inhibit EC dysfunction. One of its biomarker is activating transcription factor 6 (ATF6). However, ERS role in endothelial cells is still poorly understood. Hence we performed in vitro experiment using phenyl butyrate acid as ERS inhibitor to TNF-α -induced-ATF6 expression in human vein derived endothelial cells. We measured ATF6 expression in endothelial cells as ERS biomarker and use phenyl butyrate acid (PBA) as potent selective ERS inhibitor to block its pathway. The early result shows that PBA decrease translocated ATF6 expression in endothelial culture. From the result, it can been concluded that PBA has role in decrease endoplasmic reticulum stress in endothelial cells.