Phytochemical Content and in Vitro Antioxidant, Antibacterial and Antitumor Activities of Phoenix Dactylifera Fruit Extract
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Publication date: 2020-10-20
Eurasian J Anal Chem 2020;15(2):emEJAC201014
Phoenix dactylifera fruits are important sources for carbohydrates, minerals, vitamins, phenols and flavonoids compounds, which are important for human health. The objective of study: To evaluate the chemical composition, minerals, phytochemical, antioxidant activity using [FRAP+ and DPPH+], and antibacterial properties against some bacterial strains, (E. coli, St. aureus, L. monocytogenes and B. subtillis), of Phoenix dactylifera fruits. In addition, cytotoxic study of plant fruit extracts on human cancer cell lines (MCF-7 and HCT-116). The phytochemical screening showed that the fruit extracts contain alkaloids, flavonoids, tannins, phenols, saponins, carbohydrates and except steroids. The Phoenix dactylifera fruits have the highest value of polyphenol and flavonoid content which were 181.27mg GAE/g and 31.09mg QE/g, respectively. The antioxidant activity was evaluated using the ferric reducing antioxidant power (FRAP) radical into 79.17 and 87.05%, at a concentration 16mg/ml of aqueous and methanolic extracts. DPPH assay revealed a good antioxidant activity of methanolic extract which was (IC50 0.45μg). Moreover, the methanolic extract of Phoenix dactylifera led to the highest growth inhibition (18.75, 20.5 and 17mm), against E. coli, L. monocytogenes and B. subtillis at a concentration 4mg/ml, respectively. While, the aqueous extract provided the medium percentage of growth inhibition (11.5, 9.75 and 12.5mm) against E. coli, St. aureus and B. subtillis at a concentration 4mg/ml, respectively. Moreover, the fruit extracts have a reasonable effect on breast and colon cancer cell lines inhibition when compared with Thymoquinone at a concentration 75μg/ml.