Quantification of Baicalein, Chrysin, Biochanin-A and Ellagic Acid in Root Bark of Oroxylum indicum by RPHPLC with UV Detection
More details
Hide details
Department of Pharmacognosy, KB Institute of Pharmaceutical Education & Reasearch, Gadhinagar
Analytical Development Laboratories, Zydus Cadila Limited, Ahmedabad-sarkhej Highway, Ahmedabad
Department of Pharmacology, L. M. College of Pharmacy, Navrangpura, Ahmedabad
Online publish date: 2008-03-13
Publish date: 2008-03-13
Eurasian J Anal Chem 2008;3(2):245–257
Oroxylum indicum (Syonakh), belonging to the family Bignoniaceae, has been used for the present study. Preliminary phytochemical screening revealed the presence of phytocostituents like flavonoids, alkaloids, tannins and anthraquinone in the root bark of Oroxylum indicum. The preliminary screening using TLC technique reflected the presence of four phytoconstituents such as baicalein, chrysin, biochanin-A and ellagic acid in both petroleum ether and hydrolysed n-butanol fractions. Modern phytochemical analysis in terms of quantification of different bioactive phytoconstituents in the root bark of Oroxylum indicum was performed using reverse phase high performance liquid chromatography (RP-HPLC) fingerprint. To evaluate the quality of root bark of Oroxylum indicum, a simple rapid and accurate RP-HPLC method was developed for the assessment of four bioactive phytoconstituents viz. chrysin, baicalein, biochanin-A and ellagic acid. The components were quantified in different extracts viz. alcohol, petroleum ether, ethyl acetate and n-buatnol successively. Quantification of phytoconstituents was done by using RP-HPLC in petroleum ether and hydrolysed n-butanol fraction. Standard baicalein, chrysin biochanin-A and ellagic acid (Sigma-Aldrich) were employed for the development of the method. The RP-HPLC system used a base deactivated C18 column with water, methanol, acetonitrile and orthophosphoric acid as the mobile phase and detection was performed at 262 nm. The method was precise with relative standard deviation for these constituents that ranged between 0.5-1.0% (interday). The content of four phytoconstituents in the root bark of Oroxylum indicum was determined to establish the effectiveness of the method.
1. John, A.P. (2001) Healing Plants of Peninsular India, CAB International Wallingford, UK, pp. 169-170.
2. Anonymous. (1998) The Ayurvedic Pharmacopoeia of India. Government of India, Ministry of health and family welfare department of Indian system of medicine and Homeopathy, New Delhi, India, pp. 209-210.
3. Warrier, P.K., Nambiar, V.P.K., & Ramankutty C. (1995) In: Oroxylum indicum. Indian medicinal Plants. Vol: 4, Warrier et al. (Eds.), Orient Longman Ltd., Madras, India, pp.186-190.
4. Sankara S, Nair AGR (1972-a): Flavanoid of stem bark of Oroxylum indicum. Current Science 41:62-63.
5. Sankara S, Nair AGR (1972-b): Flavonoids from the leaves of Oroxylum indicum and Pajanelia longifolia. Phytochemistry 11: 439-440.
6. Vasanth S, Natarajan M, Sundaresan R, Bhima Rao R, Kundu AB (1991): Ellagic acid from the root bark of Oroxylum indicum. Indian Drugs 28:507.
7. Tie Hong, Guang-Bi jin, Shigefumi Cho Cyong (2002): Evaluation of the antiinflammatory effect of Baicalein in mice. Planta Medica 68: 268-271.
8. Kennouf S, Benabdallah H, Gharzouli K, Amira S, Ito H, Kim TH, Yoshida T, Gharzouli A (2003): Effect of tannins from Quercus suber and species leaves on ethanol-induced gastric lesions in mice. J Agri food chem. 51:1469-1473.
9. Ng TB, Liu F, Wang ZT (2000): Antioxidant activity of natural products from plants. Life Sciences 68:709-723.
10. Niedworok J, Jankowstia B, Kowalczy E, Okroj W (1999): A comparative investigation of hepatoprotective effects of baicalein and sylimarol. Herba Polonica 45:199-205.
11. Lien C, Lean T, Wen C, Mei-Yin C, Chun-Ching L (2003): Immunomodulatory activities of flavonoids, monoterpenoids, triterpinoids, iridoid glycosides and phenolic compounds of Plantago species. Planta Medica 69: 600-604.
12. Kujumgier A, Tsvetkoova J, Serkedjieva Y, Bankova V, Christov R, Popov S (1999): Antibacterial, antifungal and antiviral activity of propolis of different geographic origin. J Ethanopharmacology 64:235-240.
13. Tahara S, Hashihidoka Y, Mizutani (1987): Flavonoids as medicines. Agri Biol chem. 51:1039-1045.
14. Knight D, Eden J (1996): A review of the clinical effects of phytooestrogens. Obetet Gynecol 87: 897-904.
15. Jadhav P, Laddha K (2004): Estimation of gallic acid and ellagic acid from Terminalia chebula Retz. Indian drugs 41: 200-206.
16. Dornstauder E, Jisa, Utterriender I, Krenn L, Kubelka W, Jungbauer A (2001): Estrogenic activity of two standardized red clover extracts intended for large scale use in hormone replacement therapy. J Steroid Biochem Mole Biol 78: 67-75.