Quantification of Baicalein, Chrysin, Biochanin-A and Ellagic Acid in Root Bark of Oroxylum indicum by RPHPLC with UV Detection
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Department of Pharmacognosy, KB Institute of Pharmaceutical Education & Reasearch, Gadhinagar
Analytical Development Laboratories, Zydus Cadila Limited, Ahmedabad-sarkhej Highway, Ahmedabad
Department of Pharmacology, L. M. College of Pharmacy, Navrangpura, Ahmedabad
Online publication date: 2008-03-13
Publication date: 2008-03-13
Eurasian J Anal Chem 2008;3(2):245–257
Oroxylum indicum (Syonakh), belonging to the family Bignoniaceae, has been used for the present study. Preliminary phytochemical screening revealed the presence of phytocostituents like flavonoids, alkaloids, tannins and anthraquinone in the root bark of Oroxylum indicum. The preliminary screening using TLC technique reflected the presence of four phytoconstituents such as baicalein, chrysin, biochanin-A and ellagic acid in both petroleum ether and hydrolysed n-butanol fractions. Modern phytochemical analysis in terms of quantification of different bioactive phytoconstituents in the root bark of Oroxylum indicum was performed using reverse phase high performance liquid chromatography (RP-HPLC) fingerprint. To evaluate the quality of root bark of Oroxylum indicum, a simple rapid and accurate RP-HPLC method was developed for the assessment of four bioactive phytoconstituents viz. chrysin, baicalein, biochanin-A and ellagic acid. The components were quantified in different extracts viz. alcohol, petroleum ether, ethyl acetate and n-buatnol successively. Quantification of phytoconstituents was done by using RP-HPLC in petroleum ether and hydrolysed n-butanol fraction. Standard baicalein, chrysin biochanin-A and ellagic acid (Sigma-Aldrich) were employed for the development of the method. The RP-HPLC system used a base deactivated C18 column with water, methanol, acetonitrile and orthophosphoric acid as the mobile phase and detection was performed at 262 nm. The method was precise with relative standard deviation for these constituents that ranged between 0.5-1.0% (interday). The content of four phytoconstituents in the root bark of Oroxylum indicum was determined to establish the effectiveness of the method.
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