Quantitative Determination of Levofloxacin and Ambroxol Hydrochloride in Pharmaceutical Dosage Form by Reversed-Phase High Performance Liquid Chromatography
 
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1
Vinayaka Mission College of Pharmacy Salem-636008. Tamilnadu, India
2
Deputy Manager, Zydus Cadila Healthcare Limited Sarkhej Bavla N.H.-8A, Changodar, Ahmedabad, Gujarat, India
3
Senior Scientific Assistant, Analytical Development Laboratory, Zydus Cadila Limited, Ahmedabad-382 210, Gujarat, India
CORRESPONDING AUTHOR
Krupa M. Kothekar   

Vinayaka Mission College of Pharmacy, Salem-636008, Tamilnadu, India. Phone:+079-25434016, 09924724879
Online publication date: 2007-03-15
Publication date: 2007-03-15
 
Eurasian J Anal Chem 2007;2(1):21–31
 
KEYWORDS
ABSTRACT
The objective of this present work was to develop and validate analytical method for quantitative determination of Levofloxacin and Ambroxol hydrochloride in a new tablet formulation. Chromatographic separation of the two drugs were analyzed on a Hypersil BDS C18 column (25cm X 4.6mm, 5μm). The mobile phase constituted of Buffer: Acetonitirile: Methanol (650:250:100) with triethylamine and pH adjusted to 5.2 with dilute orthophosphoric acid was delivered at the flow rate 1.0 mL min-1. Detection was performed at 220 nm. Separation was completed within 10 min. Calibration curves were linear with coefficient correlation between 0.99 to 1.0 over a concentration range of 7 to 22 μg mL-1 of Levofloxacin and 50 to 150 μg/mL for Ambroxol hydrochloride respectively. The relative standard deviation (R.S.D) was found <2.0%.
 
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