RP-HPLC Determination of Nitazoxanide in Bulk and Different Tablet Formulations
 
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A.I.S.S.M.S. College of Pharmacy, Kennedy Road, Near R.T.O., Pune - 411 001
Online publication date: 2008-11-01
Publication date: 2008-11-01
 
Eurasian J Anal Chem 2008;3(3):318–323
 
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ABSTRACT
A simple, accurate and precise validated RP-HPLC method for determination of Nitazoxanide has been developed. Analysis was carried out on Jasco HPLC system with HiQ-sil C18 column (250 x 4.6 mm i.d.) using Acetonitrile: 0.005 mol.L–1 Tetrabutyl ammonium hydrogen sulphate in ratio of 55:45 v/v as mobile phase and Satranidazole as an internal standard The detection was carried out using UV detector set at 240 nm. For this method, Beer’s law is obeyed in the concentration range of 5.0 to 30.0 μg mL–1 of Nitazoxanide. The developed method has been successfully applied for the analysis of drug in bulk and pharmaceutical formulations. The mean percent recoveries were found to be 100.19 ± 0.584 for Brand 1 and 100.26 ± 1.1341 for Brand 2. The method was validated with respect to linearity, precision and accuracy as per the International Conference on Harmonisation (ICH) guidelines.
 
REFERENCES (7)
1.
http://www.drugs.com/MTM/nitaz... Accessed on January 30, 2007.
 
2.
http://www.rxlist.com/cgi/gene... Accessed on January 30, 2007.
 
3.
http://www.nlm.nih.gov/medline... Accessed on January 30, 2007.
 
4.
http://www.centerwatch.com/pat... Accessed on January 30, 2007.
 
5.
Kapse G K, Prabhakar G, Raju S, Appala (2006) Spectrophotometric methods for the estimation of nitazoxanide in pharmaceutical formulations. IJPS 68:403.
 
6.
Prabhakar G, Kapse G K, Appala R (2006) Spectrophotometric determination of nitazoxanide in pharmaceutical Dosage form. The Indian Pharmacist 54:83.
 
7.
ICH Harmonised Tripartite Guideline (Nov 2005) Validation of Analytical Procedures: Text and Methodology Q2 (R1).
 
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