RP-HPLC Method Development and its Validation for Quantitative Determination of Rimonabant in Human Plasma
 
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1
School of Pharmaceutical Sciences, Rajiv Gandhi Technological University, Gandhi Nagar, Bhopal, MP 462 036, India
2
Sagar Institute of Research and Technology, Bhopal, 462 036, MP, India
3
LBS College of Pharmacy, Jaipur, Rajasthan, 302 004, India
CORRESPONDING AUTHOR
Deepti Jain   

School of Pharmaceutical Sciences, Rajiv Gandhi Technological University, Gandhi Nagar, Bhopal, MP 462 036, India
Publish date: 2017-11-02
 
Eurasian J Anal Chem 2012;7(3):115–122
KEYWORDS
ABSTRACT
A simple, accurate and precise HPLC method was developed and validated for determination of rimonabant in human plasma. Following liquid-liquid extraction, chromatographic separation was accomplished using C18 column with mobile phase consisting of acetonitrile: water (90:10, v/v), drug was detected at 260 nm. The LOD and LOQ were 3.3 and 9.9 ng mL-1, respectively. The method is linear in range 50-1000 ng mL-1. The average extraction recovery of drug from plasma was found 78.45%. The precision of method was found less than 2.8%, and accuracy was found between 97.12-101.71%. The assay may be applied to a pharmacokinetic and bioequivalence study of rimonabant.
 
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