RESEARCH PAPER
Simultaneous Determination Of Levetiracetam And Preservatives In Oral Solution Formulation Using Hplc-Uv Method With A Programble Detection Wavelength
Ali Fouad 1  
 
 
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Pharmaceutical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, Egypt
CORRESPONDING AUTHOR
Ali Fouad   

Pharmaceutical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, 71524, Assiut, Egypt
Publish date: 2019-11-06
 
Eurasian J Anal Chem 2019;14(4):emEJAC-00342
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ABSTRACT
Background:
A reversed-phase high performance liquid chromatography (RP-HPLC) method was developed and validated for the simultaneous determination of levetiracetam, methylparaben and propylparaben in levetiracetam oral solution formulation.

Material and methods:
The separation was achieved on Equisil BDS, C18, 5 µm, (150 mm × 4.6 mm) using 1.4 g/L of NaH2PO4 : Methanol in ratio (55:45) respectively pH 7.7 by NaOH as mobile phase and at a flow rate of 1.0 mL/min.

Results:
Detection was carried out using a UV detector Start with 240 nm then at 9 minutes change to 254 nm. The total chromatographic analysis time per sample was about 14 min.

Conclusions:
Analytical parameters system suitability, specificity, linearity, precision, repeatability accuracy, LOD/LOQ and stability of standard solution were determined by validation procedure and found to be satisfactory.

eISSN:1306-3057