Simultaneous Determination of Paracetamol and Piroxicam in Tablets by Thin Layer Chromatography Combined with Densitometry
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Department of Pharmaceutical Chemistry, R. C. Patel College of Pharmacy, Shirpur, Dist: Dhule, (M.S.), India 425 405
Atul A. Shirkhedkar   

Department of Pharmaceutical Chemistry, R. C. Patel College of Pharmacy, Shirpur, Dist: Dhule, (M.S.), India 425 405
Online publication date: 2008-03-13
Publication date: 2008-03-13
Eurasian J Anal Chem 2008;3(2):258–267
A simple, rapid and accurate High-performance thin-layer chromatography (HPTLC) method has been established and validated for the simultaneous determination of paracetamol and piroxicam in tablets. The method is based on HPTLC separation of the two drugs followed by densitometric measurements of their spots at 288 nm. The separation was carried out on Merck TLC aluminium sheets of silica gel 60F-254 using n-Dichloroethane: methanol: triethylamine (10:2.5:1, v/v) as a mobile phase. Calibration curves were linear in range of 1.625 - 14.625 μg/spot and 0.1 - 0.9 μg/spot for paracetamol and piroxicam, respectively. Method was successively applied to tablet formulation. No chromatographic interferences from the tablet excipients were found. The method was validated in accordance with the requirements of ICH guidelines.
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