Simultaneous Determination of the Main Organic Acids in Anatolian Black Pine by HPLC with DAD Detector
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Bozok University, Faculty of Arts and Sciences, Department of Chemistry, Yozgat, Turkey
Mohammed Akkbik   

Bozok University, Faculty of Arts and Sciences, Department of Chemistry, Yozgat, Turkey
Publication date: 2016-07-28
Eurasian J Anal Chem 2016;11(4):181–195
The versatile characterization of organic materials in Anatolian black pine (Pinus nigra) especially the significant organic acids is great importance. A rapid, simple, and sensitive HPLC–DAD method was developed and presented for a simultaneous detection of six organic acids. This method was thoroughly validated to determine the most abundant organic acids present in Anatolian black pine and their distribution in branches, leaves and cones. Separation of six organic acids were developed and achieved on ODS C18 column (250mm×4.6mm, 5μm particle size), using a mixture of (A) water (pH: 2.0, adjusted by H2SO4) and (B) acetonitrile in the ratio of (95:5, v/v) at wavelength of 210 nm with a flow rate of 0.8 mL min-1 within 14 minutes. The dynamic range was between 5 to 1000 mg L-1 with relative standard deviation less than 0.73%, (n=4). Limits of detection and the recoveries for oxalic acid, tartaric acid, formic acid, lactic acid, acetic acid and citric acid were ranged 1.85 mg L-1 (87.75-99.36%), 3.61 mg L-1 (100.89-119.55%), 4.01 mg L-1 (83.57-119.64%), 1.16 mg L-1 (82.29-112.19%), 1.19 mg L-1 (80.86-110.42%) and 2.87 mg L-1 (96.96-117.74%), respectively.
Kaya Z and Temerit A (1993). Genetic structure of marginally located Pinus nigra var pallasiana populations in central Turkey. Silvae Genet 43, 272.
Gülçin I, Büyükokuroğlu ME, Oktay M and Küfrevioğlu ÖI (2003). Antioxidant and analgesic activities of turpentine of Pinus nigra Arn subsp pallsiana (Lamb) Holmboe. Ethnopharmacology 86, 51.
Ucar G and Balaban M (2004). Volatile needle extractives of Anatolian black pine varieties: P. nigra subsp. pallasiana var. pallasiana and var. Pyramidata. Biochemical Systematics and Ecology 32, 983.
Köse N, Akkemik Ü, Dalfes HN, Özeren MS and Tolunay D (2012). Treering growth of Pinus nigra Arn. subsp. pallasiana under different climate conditions throughout western Anatolia. Dendrochronologia 30, 295.
Ustun O, Senol FS, Kurkcuoglu M, Orhan IE, Kartal M, Baser KHC (2012). Investigation on chemical composition, anticholinesterase and antioxidan activities of extracts and essential oils of Turkish Pinus species and pycnogenol. Industrial Crops and Products 38, 115.
Kesselmeier J, Bode K, Hofmann U, Müller H, Schafer L, Wolf A, Ciccioli P, Brancaleoni E, Cecinato A, Frattoni M, Foster P, Ferrari C, Jacob V, Fugit JL, Dutaur L, Simon V and Torres L (1997). Emission of short chained organic acids, aldehydes and monoterpenes from quercus ilex l and pinus pinea l in relation to physiological activities, carbon budget and emission algorithms. Atmospheric Environment 31, 119.
Ahonen-Jonnarth U, Hees HJPAWV, Lundström U and Finlay R (2000). Organic acids produced by mycorrhizal pinus sylvestris exposed to elevated aluminium and heavy metal concentrations. New Phytologist.146, 557.
Hsieh LY, Kuo SC, Chen CL and Tsai YI (2009). Size distributions of nano micron dicarboxylic acids and inorganic ion in suburban PM episode and nonepisodic aerosol. Atmospheric Environment 43, 4396.
Kuo SC, Tsai YI, Tsai CH, Hsieh LY (2011). Carboxylic acids in PM2.5 over Pinus morrisonicola forest and related photoreaction mechanisms identified via Raman spectroscopy. Atmospheric Environment 45, 6741.
Shi S, Condron L, Larsen S, Richardson AE, Jones E, Jiao J, Callaghan MO and Stewart A (2011). In situ sampling of low molecular weight organic anions from rhizosphere of radiata pine (Pinus radiata) grown in a rhizotron system. Environmental and Experimental Botany 70, 131.
Wang M, Qu F, Shan XQ and Lin JM (2003). Development and optimization of a method for the analysis of low-molecular-mass organic acids in plants by capillary electrophoresis with indirect UV detection. Journal of Chromatography A 989, 285.
Pullman GS and Buchanan M (2006). Identification and quantitative analysis of stage specific organic acids in loblolly pine (Pinus taeda L.) zygotic embryo and female gametophyte. Plant Science 170, 634.
Gode F, Atalay ED and Pehlivan E (2008). Removal of Cr (VI) from aqueous solutions using modified red pine sawdust. Journal of Hazardous Materials 152, 1201.
Aguiar A, Souza-Cruz PBD and Ferraz A (2006). Oxalic acid Fe3+ reduction activity and oxidative enzymes detected in culture extracts recovered from Pinus taeda wood chips biotreated by Ceriporiopsis subvermispora. Enzyme and Microbial Technology 38, 873.
Zhang A, Fang YL, Meng JF, Wang H, Chen SX and Zhang ZW (2011). Analysis of low molecular weight organic acids in several complex liquid biological systems via HPLC with switching detection wavelength. Journal of Food Composition and Analysis 24, 449.
Nergiz C and Ergönül PG (2009). Organic acid content and composition of the olive fruits during ripening and its relationship with oil and sugar. Scientia Horticulturae 122, 216.
Albert DB and Martens CS (1997). Determination of low molecular weight organic acid concentrations in seawater and pore water samples via HPLC. Marine Chemistry 56, 27.
Cunha SC, Fernandes JO and Ferreira IMPLVO (2002). HPLC/UV determination of organic acids in fruit juices and nectars. European Food Research and Technology 214, 67.
Shui G and Leong LP (2002). Separation and determination of organic acids and phenolic compounds in fruit juices and drinks by high-performance liquid chromatography. Journal of Chromatography A 977, 89.
Kerem Z, Bravdo BA, Shoseyov O and Tugendhaft Y (2004). Rapid liquid chromatography ultraviolet determination of organic acids and phenolic compounds in red wine and must. Journal of Chromatography A 1052, 211.
Kakola J, Alen R, Pakkanen H, Matilainen R and Lahti K (2007). Quantitative determination of the main aliphatic carboxylic acids in wood kraft black liquors by high performance liquid chromatography mass spectrometry. Journal of Chromatography A 1139, 263.
Quiros ARBD, Lage-Yusty MA and Lopez-Hernandez J (2009). HPLC analysis of organic acids using a novel stationary phase. Talanta 78, 643.
Hees PAWV, Dahlen J, Lundstrom US, Boren H and Allard B (1999). Determination of low molecular weight organic acids in soil solution by HPLC. Talanta 48, 173.
Song D and Wang J (2003). Modified resolution factor for asymmetrical peaks in chromatographic separation. Journal of Pharmaceutical and Biomedical Analysis 32, 1105.
Wang X, Kang J, Wang S, Lu JJ and Liu S (2008). Chromatographic separations in a nanocapillary under pressure-driven Conditions. Journal of Chromatography A 1200, 108.
Sankalia JM, Sankalia MG, Sutariya VB and Mashru RCJ (2007). Nateglinide quantification in rabbit plasma by HPLC: Optimization and application to pharmacokinetic study. Journal of Pharmaceutical and Biomedical Analysis 44, 196.
Scherer R, Rybka ACP, Ballus CA, Meinhart AD, Filho JT and Godoy HT (2012). Validation of a HPLC method for simultaneous determination of main organic acids in fruits and juices. Food Chemistry 135, 150.
Kelebek H, Selli S, Canbas A and Cabaroglu T (2009). HPLC determination of organic acids, sugars, phenolic compositions and antioxidant capacity of orange juice and orange wine made from a Turkish cv. Kozan. Microchemical Journal 91, 187.
Castellari M, Versari A, Spinabelli U, Galassi S and Amati A (2000). An improved HPLC method fort he analysis of organic acids, carbohydrates and alcohols in grape musts and wines. Journal of Liquid Chromatography & Related Technologies 23(13), 2047.
Rodrigues CI, Marta L, Maia R, Miranda M, Ribeirinho M and Maguas C (2007) Application of solid-phase extraction to brewed coffee caffeine and organic acid determination by UV/HPLC. Journal of Food Composition and Analysis 20, 440.
Chinnici F, Spinabelli U, Riponi C and Amati A (2005). Optimization of the determination of organic acids and sugars in fruit juices by ion-exclusion liquid chromatography. Journal of Food Composition and Analysis 18, 121.
Eyeghe-Bickong HA, Alexandersson EO, Gouws LM, Young PR and Vivier MA (2012). Optimisation of an HPLC method for the simultaneous quantification of the major sugars and organic acids in grapevine berries. Journal of Chromatography B 885– 886, 43.