Simultaneous Estimation of Cilnidipine and Valsartan by RP-HPLC in Tablet Formulation
 
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1
Jawaharlal Nehru Technological University, India
2
Government College of Pharmacy Aurangabad, India
3
ARA College of Pharmacy, India
CORRESPONDING AUTHOR
Ramanlal Narayanrao Kachave   

Jawaharlal Nehru Technological University Kakinada-Andhra Pradesh & Amrutvahini College of Pharmacy, Amrutnagar, P.O. Sangamner (S.K.), India
Publication date: 2016-08-07
 
Eurasian J Anal Chem 2016;11(5):245–253
 
KEYWORDS
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ABSTRACT
A new high performance liquid chromatography method was developed and validated for the quantitation of Cilnidipine and Valsartan in pharmaceutical formulations. Determination was performed using an ODS C18, 250mm x 4.6mm, 5μm column, a mobile phase containing Methanol: Water (85:15) pH 3 adjusts with ortho-phosphoric acid in isocratic flow rate 1.0 mLmin-1. The method was validated with respect to linearity, precision, robustness, and accuracy. The calibration graphs ranged from 1-5 μg/mL in Cilnidipine and 8-40 μg/mL Valsartan Intra- and interday relative standard deviation values for the standard solutions were 0.5%, 1.64% and 0.22%, 1.62 %. Robustness of relative standard deviation values was 0.334, 0.101 respectively. Total recoveries of Cilnidipine and Valsartan from the laboratory prepared mixtures were 98.94 % and 99.04 % respectively.
 
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