Simultaneous Spectrophotometric Estimation of Lercanidipine Hydrochloride and Atenolol in Tablet Dosage Form
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Sagar Institute of Research and Technology-Pharmacy, Bypass Road, Bhopal – 462036, India
Truba Institute of Pharmacy, Bhopal (M.P.), India
Nilesh Jain   

Sagar Institute of Research and Technology-Pharmacy, Bypass Road, Bhopal – 462036, India
Publish date: 2017-10-15
Eurasian J Anal Chem 2011;6(2):84–90
Two accurate, precise, sensitive and economical spectrophotometric methods were developed and validated for simultaneous estimation of Lercanidipine hydrochloride and Atenolol in tablet dosage form. These methods were developed based on the simultaneous estimation of drugs in a binary mixture without previous separation. The methods employed were Absorbance Ratio Method (QAnalysis) (I) and Simultaneous Equation Method (Vierodt’s Method) (II). The first method employs 261nm as λ1 (Isobestic point) and 273 nm as λ2max of Atenolol) for formation of equations. The second method employs estimation of a drug concentration by selecting λmax where the absorbances of these drugs were maximum. So λmax for Lercanidipine hydrochloride and Atenolol is 242 nm and 273 nm respectively. Lercanidipine hydrochloride and Atenolol obey Beer’s law in the concentration range 10-50 μg mL-1 (r2=0.9999) and 50-250 μg mL-1 (r2=0.9999) in 0.1 N HCL. The mean recovery for Lercanidipine hydrochloride and Atenolol were found to be 98.07±0.21% and100±0.14% from method I and 98.60±0.36% and 100±0.05% from method II. The developed methods were validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values. Thus the proposed methods were successfully applied for simultaneous determination of Lercanidipine hydrochloride and Atenolol in routine industrial work.
Oneil M J, Smith A and Heckelman P E (2001) The Merck Index, 13th edition, Merck Research Laboratories, Whitehouse Station: New Jersey, p. 5465, 863.
Sweetman S C (2002) Martindale: The complete drug reference, 33rd edition, The Pharmaceutical Press, London, p. 897-3, 825-3.
Christians T, Diewald D, Wessler C, Otte Y, Lehmann J and Holzgrabe U (1999) The voltametric polarographic method to determine lercanidipine. J Chromatogr A 853: 455-60.
Alvarez L A, Nunez Vergara L J, Pujol S and Squella A (2002) Voltametric behavior of lercanidipine and its differential pulse palarographic determination in tablets. Electroanalysis 14: 1098-1104.
Mubeen G, Damanjit S G and Kadri U (2008) Spectrophotometric method for estimation of Lercanidipine in tablets. J Biomed Pharmcol 1(2):479-80.
Eswar G M, Shyam S B and Devala R G (2004) Spectrophotometric methods for the determination of lercanidipine in pharmaceutical formulations. The Indian Pharmacist (29):77-78.
Abu El-Enin M A, El-Wasseef D R, El-Sherbiny D T and El-Ashry S M (2009) Spectrophotometric determination of labetalol and lercanidipine in pure form and in pharmaceutical preparations using ferric-1,10-phenanthroline. Int J Biomed Sci 5(3): 261-266.
Salem I I, Idrees J, Tamimi J A T and Farina P (2004) Selective and rapid liquid chromatography mass spectrometry method for determination of lercanidipine in human plasma. J Chromatogr A 803: 201-207.
Jessica F, Roberto G, Carlo B and Vanni C (2006) Photochemical stability of lercanidipine and its determination in tablets by HPLC-UV and LC-ESI-MS/MS. J Pharm Biomed Anal 41:176-181.
Alvarez L A, Pujol S, Squella J A and Nunez Vergara L J (2003) A selective HPLC method for determination of lercanidipine HCl in tablet. J Pharm Biomed Anal 31: 1-9.
Vijaya M K and Muley P R (2004) Reverse phase high performance liquid chromatographic method for determination of lercanidipine in bulk drug and solid dosage forms. Indian Drugs 41 (1):24-27.
Kalovidouris M, Michalea S, Robola N, Koutsopoulou M and Panderi I (2006) Ultra-performance liquid chromatography/tandem mass spectrometry method for the determination of lercanidipine in human plasma. R Comm M Spectra 20 (19): 2939–2946.
Sivakumar T, Venkatesan P, Manavalan R and K Valliappan (2007) Development of a HPLC method for the simultaneous determination of losartan potassium and atenolol in tablets. Indian J Pharma Sci 69 (1):154-157.
Ravi Sankar S, Nanjan M J, Vasudevan M, Shaat N, Suresh B and Sankar S R (1997) Simultaneous estimation of atenolol and amlodipine in formulations by reversed Phase-HPLC. Indian J Pharma Sci 59(4):171-173.
Shah S A, Rathod I S, Shishoo C J, Savale S S, Satia M C and Bhat K M.(2000) A sensitive high performance liquid chromatography method for determination of bioequivalence of atenolol formulations. Indian J Pharma Sci 62(3): 187-192.
Lamprecht G, Kraushofer T, Stoschitzky K and Lindner W (2000) Enantioselective analysis of (R)- and (S)-atenolol in urine samples by a high-performance liquid chromatography column-switching setup. J Chromatogr B 740: 219.
Shafaati A and Clark B J (1996) Development and validation of a capillary zone electrophoresis for determination of atenolol in presence of its related substances in bulk and tablet dosage form. J Pharm Biomed Anal 14: 1547.
Prasad U V, Rao G V and Sastry C S (2002) New spectrophotometric analysis of atenolol in pure and pharmaceutical formulations. Indian J Pharma Sci 64 (4):129-132.
Kasture A V and Madhuri R (2006) Simultaneous UV-spectrophotometric method for the estimation of atenolol and amlodipine Besylate in combined dosage form. Indian J Pharma Sci 68(3):394-396.
Prasad C V N, Parihar C, Sunil K and Parimoo P (1998) Simultaneous determination of amiloride HCl hydrochlorothiazide and atenolol in combined formulations by derivative spectroscopy. J Pharm Biomed Anal.17:877-84.
International conference on harmonization of technical requirements for regression of pharmaceutical for human use. 2005 Validation of analytical procedures: text and methodology Q2 (R1).