Spectrophotometric Determination of Five Commercial Drugs in Pure Form and Pharmaceutical Formulations by Ion-Pair Complexation with Alizarin Red S
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Department of Chemistry, School of Sciences, Gujarat University, Navrangpura, Ahmedabad- 380009, Gujarat, India
Department of Chemistry, St. Xavier’s College, Navrangpura, Ahmedabad-380009, Gujarat, India
Pranav S. Shrivastav   

Department of Chemistry, School of Sciences, Gujarat University, Navrangpura, Ahmedabad- 380009, Gujarat, India
Publication date: 2017-10-05
Eurasian J Anal Chem 2015;10(2):68–83
Simple, accurate, selective and rapid spectrophotometric methods have been developed for the determination of itopride, midodrine, diclofenac, mesalamine and sumatriptan in their pure as well as in pharmaceutical dosage forms. The method was based on ion-pair complex formation between the drugs and anionic dye, alizarin red S in an acidic medium (pH 2.0-4.0). The complexes formed were quantitatively extracted into chloroform and measured at 284, 287, 279, 285 and 280 nm wavelength for itopride, midodrine, diclofenac, mesalamine and sumatriptan respectively. Beer’s law was obeyed in the concentration range of 3.5-35 µg mL-1 for itopride and diclofenac, 2.5-180 µg mL-1 for midodrine, 3.0-80 µg mL-1 for mesalamine and 4.0-200 µg mL-1 for sumatriptan. The stoichiometry of the complexes formed between the drugs and the dye was 1:1 as determined by Job’s method of continuous variation. The association constant (KIP) of the ion-pair complexes formed was evaluated using Benesi-Hildebrand equation. Limit of detection, limit of quantification and Sandell’s sensitivity of the methods were also estimated. The proposed methods were successfully employed for the determination of these drugs in their pharmaceutical dosage forms.
Siddiqui MR, Alothman ZA and Rahman N (2013) Analytical techniques in pharmaceutical analysis: A review. Arab J Chem DOI: 10.1016/j.arabjc.2013.04.016.
Gouda AA, Kotb El-Sayed MI, Amin AS and El Sheikh R (2013) Spectrophotometric and spectrofluorometric methods for the determination of non-steroidal antiinflammatory drugs: A review. Arab J Chem 6: 145.
Rojas FS and Ojeda CB (2009) Recent developments in derivative ultraviolet/visible absorption spectrophotometry: 2004–2008: A review. Anal Chim Acta 635: 22.
S Yoon S, Lee H, Kim TE, Lee S, Chee DH, Cho JY, Yu KS and Jang IJ (2014) Comparative steady-state pharmacokinetic study of an extended-release formulation of itopride and its immediate-release reference formulation in healthy volunteers. Drug Des Devel Ther 8: 123.
Huang X, Lv B, Zhang S, Fan YH and Meng LN (2012) Itopride therapy for functional dyspepsia: A meta-analysis. World J Gastroenterol 18: 7371.
Izcovich A, Gonzalez Malla C, Manzotti M, Catalano HN and Guyatt G (2014) Midodrine for orthostatic hypotension and recurrent reflex syncope: a systematic review. Neurology 83: 1170.
Biaggooni I (2014) New developments in the management of neurogenic orthostatic hypotension. Curr Cardiol Rep 16: 542.
Dastidar SG, Ganguly K, Chaudhuri K and Chakrabarty AN (2000) The anti-bacterial action of diclofenac shown by inhibition of DNA synthesis. Int J Antimicrob Agents 14: 249.
Nanda K and Moss AC (2012) Update on the management of ulcerative colitis: treatment and maintenance approaches focused on MMX® mesalamine. Clin Pharmacol Adv App 4: 41.
Sandborn WJ, Feagan BG and Lichtenstein GR (2007) Medical management of mild to moderate Crohn's disease: evidence-based treatment algorithms for induction and maintenance of remission. Alimentary Pharmacol Ther 26: 987.
Deleu D and Hanssens Y (2000) Current and emerging second-generation triptans in acute migraine therapy: a comparative review. J Clin Pharmacol 40: 687.
Khoury CK and Couch JR (2010) Sumatriptan-naproxen fixed combination for acute treatment of migraine: a critical appraisal. Drug Des Devel Ther 4: 9.
Zate SU, Kothawade PI, Gajbe JW, Pramod AS and Boraste SS (2010) Spectrophotometric method development and validation of itopride hydrochloride in bulk and dosage form. Int J Drug Deliv 2: 340.
Gupta KR, Joshi RR, Chawla RB and Wadodkar SG (2010) UV spectrophotometric method for the estimation of itopride hydrochloride in pharmaceutical formulation. E-J Chem 7: S49.
Choudhary B, Goyel A and Khokra SL (2009) New visible spectrophotometric method for estimation of itopride hydrochloride from tablets formulations using methyl orange reagent. Int J Pharmacy Pharm Sci 1: 159.
Thiruvengada RV, Mohamed STs, Ramkanth S, Alagusundaram M, Ganaprakash K and Madhusudhana CC (2010) A simple RP-HPLC method for quantitation of itopride hcl in tablet dosage form. J Young Pharm 2: 410.
Suganthi A, John S and Ravi TK (2008) Simultaneous HPTLC determination of rabeprazole and itopride hydrochloride from their combined dosage form. Ind J Pharm Sci 70: 366.
Walash MI, Ibrahim F, Eid MI and Abass SAEL (2013) Stability-Indicating Spectrofluorimetric method for determination of itopride hydrochloride in raw material and pharmaceutical formulations. J Fluoresc 23: 1293.
Khalil S (2013) Micro-determination of itopride hydrochloride in pharmaceutical formulations and urine samples using icp-atomic emission spectrometry. Int J Pharm Bio Sci 4: 786.
Ptácek P, Klíma J and Macek J (2009) Optimized method for the determination of itopride in human plasma by high-performance liquid chromatography with fluorimetric detection. J Chromatogr B 877: 842.
Sattar OA, Rezk MR, Badawy AM and Khattab OM (2013) Selective determination of midodrine hydrochloride in the presence of its acidic degradation product. Anal Chem Indian J 12: 182.
El-Gindy A, Sallam S and Abdel-Salam RA (2008) Spectrophotometric method for determination of midodrine hydrochloride using ninhydrin in pharmaceutical formulations. Anal Chem Indian J 7: 529.
Elzanfaly ES, Zaazaa HE and Merey HA (2013) Ion Selective phosphotungestate and β-cyclodextrin based membrane electrodes for stability-indicating determination of midodrine hydrochloride. Acta Chim Slov 60: 256.
Yoshida H, Ohno Y, Yoshikuni K, Todoroki K, Nohta H and Yamaguchi M (2003) Determination of midodrine in human plasma by high-performance liquid chromatography with fluorescence detection. Anal Sci 19: 317.
Matin AA, Farajzadeh MA and Jouyban A (2005) A simple spectrophotometric method for determination of sodium diclofenac in pharmaceutical formulations. Il Farmaco 60:855.
Khaskheli AR, Sirajuddin S, Abro K, Sherazi STH, Afridi HI, Mahesar SA and Saeed M (2009) Simpler and faster spectrophotometric determination of diclofenac sodium in tablets, serum and urine samples. Pak J Anal Environ Chem 10: 53.
Lala LG, D’Mello PM and Naik SR (2002) HPTLC determination of diclofenac sodium from serum. J Pharm Biomed Anal 29: 539.
Mazurek S and Szostak R (2008) Quantitative determination of diclofenac sodium in solid dosage forms by FT-Raman spectroscopy. J Pharm Biomed Anal 48: 814.
Hosseinzadeh R, Maleki R and Matin AA (2007) Interaction of diclofenac with bovine serum albumin investigated by diclofenac-selective electrode. Acta Chim Slov 54: 126.
Abdel-Hamid ME, Novotny L and Hamza H (2001) Determination of diclofenac sodium, flufenamic acid, indomethacin and ketoprofen by LC-APCI-MS. J Pharm Biomed Anal 24: 587.
Darak V, Karadi AB, Arshad M and Raju SA (2011) Simple extractive spectrophotometric determination of mesalamine by acid-dye complexation methods in solid dosage form. Res J Pharmacy Tech 4: 962.
Chandra BS, Bhogela SS, Shaik M, Vadlamudi CS, Chappa M and Maddirala NS (2011) Simple and sensitive spectrophotometric methods for the analysis of mesalamine in bulk and tablet dosage forms. Quim. Nova 34: 1068.
Jejurkar L, Tapar KK, Game MD, Birari T and Ghenge G (2011) Development and Validation of Spectrophotometric Method for Mesalamine in Pure and In Tablets. Res J Pharmacy Tech 4: 589.
Zakaria RA (2013) Spectrophotometric Determination of mesalazine by 8-hydroxy quinoline and N-(1-naphthyl)ethylenediamine dihydrochloride reagents in bulk and capsule dosage forms. Raf J Sci 24: 146.
Sama NS, Gurupadayya BM and Kumar A (2011) Spectrophotometric determination of mesalamine by PDAC and NQS reagents in bulk and tablet dosage form. J Pharmacy Res 4: 39.
Hamdon EA, Zakaria SA and AL-Hamdany NM (2012) Spectrophotometric determination of mesalazine with phloroglycinol in pharmaceutical preparation. Tikrit J Pure Sci 17: 133.
Reddy MP, Prabhavathi K, Reddy NR and Reddy PR (2011) Two simple spectroscopic methods for the estimation of mesalamine in bulk sample and its pharmaceutical dosage forms. Global J Pharmacol 5: 101.
Singh RK, Patel PS and Gupta P (2010) UV spectrophotometric method for the estimation of mesalazine in bulk and its pharmaceutical dosage forms. Int J Pharm Sci Res 1: 44.
Jejurkar L and Jejurkar A (2012) HPLC method of detection for 5ASA in pure and in tablets. J Pharm Sci Tech 4: 792.
Shah CR, Suhagia BN, Shah NJ and Shah RR (2008) Development and validation of a HPTLC method for the estimation of sumatriptan in tablet dosage forms. Indian J Pharm Sci 70: 831.
Sharma MC (2013) Validated Densitometric Method for the quantification of mesalamine in pharmaceutical dosage form. World Appl Sci J 28: 1098.
Ge Z, Tessier E, Neirinck L and Zhu Z (2004) High performance liquid chromatographic method for the determination of sumatriptan with fluorescence detection in human plasma. J Chromatogr B 806: 299.
Ramu BK, Rupakumari G, Ramarao K and Raghubabu K (2011) Development of new visible spectrophotometric methods for quantitative determination of sumatriptan succinate based on charge-transfer complex formation. Int J Pharmacy Pharm Sci Res 2: 47.
Ramu BK and Raghubabu K (2011) Estimation of sumatriptan succinate in bulk and formulations by visible spectrophotometry using aromatic aldehydes. Int J Appl Bio Pharm Tech 2: 86.
Ramu BK and Raghubabu K (2011) Extractive visible spectrophotometric assay for sumatriptan succinate estimation based on ion-association complex formation. Res J Pharmacy Tech 4: 1212.
Ramu BK and Raghubabu K (2011) Ion association method for the determination of sumatriptan succinate from tablet dosage forms using tropaeolin OOO. Int J Pharmacy Pharm Sci 3: 175.
Prashanth KN, Basavaiah K and Raghu MS (2013) Utilization of N-bromosuccinimide as a brominating agent for the determination of sumatriptan succinate in bulk drug and tablets. Int J Anal Chem 2013: Article ID 934357.
Ramu BK and Raghubabu K (2010) Visible spectrophotometric determination of sumatriptan succinate in tablet dosage forms using folin reagent. Int J Pharm Biomed Sci 1: 49.
Job P (1928) Formation and stability of inorganic complexes in solution. Anal Chem 9: 113.
Benesi HA and Hildebrand JH (1949) A spectrophotometric investigation of the interaction of iodine with aromatic hydrocarbons. J Am Chem Soc 71: 2703.
Shrivastava A and Gupta VB (2011) Methods for the determination of limit of detection and limit of quantitation of the analytical methods. Chron Young Sci 2: 21.
ICH Harmonised Tripartite Guideline (2005) Validation of analytical procedures: text and methodology- Q2(R1).