Spectrophotometric Determination of Iodine Species in Table Salt, Pharmaceutical Preparations and Sea Water
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Department of Chemistry, Stella Maris College, Chennai 600 086, India
Mary George   

Department of Chemistry, Stella Maris College, Chennai 600 086, India
Publication date: 2017-10-17
Eurasian J Anal Chem 2011;6(2):129–139
A sensitive spectrophotometric method for the determination of iodine species like iodide, iodine, iodate and periodate is described. The method involves the oxidation of hydroxylamine to nitrite with iodate under acidic condition. The formed nitrite is determined based on the diazo coupling reaction between p-nitroaniline and N-(1-naphthyl) ethylenediammine dihydrochloride [NEDA]. The method obeys Beer’s law in the concentration range 1-15 μg of iodate in an overall aqueous volume of 10 mL at 545 nm and the color is stable for 3h. The relative standard deviation is 1.7 % (n=10) at 12 μg of iodate. The molar absorptivity is calculated to be 8.33 x 104 L mol-1cm-1 with a correlation coefficient of 0.9998. The developed method can be applied to samples containing iodine and iodide by oxidation to iodate using bromine solution under acidic condition. Periodate is determined by prereduction to iodate using ethylene glycol under acidic condition. The optimum experimental reaction conditions are evaluated. The effect of interfering ions on the determination of iodate is described. The proposed method has been successfully applied to the determination of iodide and iodate in salt samples, iodine in pharmaceutical preparations and added periodate in sea water sample.
Gilman A G, Goodman L S, Rad T W and Murad F (1985) The Pharmacological Basis of Therapeutics, 7th edition, Macmillan, New York.
Trivedi R H, Mehta S H, Bhatt S D and Choudhri B P (1987) Colorimetric Determination of Iodate in Iodated Salt Using P-Phenylenediamine. Ind J Technol 25: 43.
Rodeheaver G, Bellamy W, Kody M, Spatafora G, Fitton L, Leyden K and Edlich R (1982) Bactericidal Activity and Toxicity of Iodine-Containing Solutions in Wounds. Arch Surg 117(2): 181.
Edmonds J S and Morita M (1998) The Determination of Iodine Species in Environmental and Biological Samples. Pure Appl Chem 70: 1567.
Alegret S, Florido A, Lima JLFC and Machado AASC (1989) Flow-Through Tubular Iodide and Bromide Selective Electrodes Based on Epoxy Resin Heterogeneous Membranes. Talanta 8: 825.
Crecelius EA (1975) Determination of Total Iodine in Milk by X-Ray Fluorescence Spectrometry and Iodide Electrode. Anal Chem 47: 2034.
Vanhoe H F, van Allemeersch, Versieck J and Dams R (1993) Effect of Solvent Type on the Determination of Total Iodine in Milk Powder and Human Serum by Inductively Coupled Plasma Mass Spectrometry. Analyst 118: 1015.
Schrame P and Hasse S (1994) Iodine Determination in Biological Materials by ICPMS. Microchim Acta 116: 205.
Rubio S and Perez-Bendito D (1989) Micellar Media in Kinetic Determinations. Anal Chim Acta 224: 185.
Kennedy R O, Bator J, Reading C (1989) A Microassay for The Determination of Iodide and Its Application to The Measurement of The Iodination of Proteins and The Catalytic Activities of Iodo Compounds, Anal. Biochem 179:139.
Garcia M.S, Sanchez-Pedreno C, Albero M.I and Sanchez C (1991) Kinetic Determination of Iodide in Pharmaceutical and Food Samples. Analyst 116: 653.
Yonehava N, Yamana T, Tomiyasu T and Sakamoto H (1989) Simultaneous Spectrophotometric Determination of Traces of Bromide and Iodide Based on Their Catalytic Effects on Pyrocatechol Violet-Hydrogen Peroxide Reaction. Anal Sci 5:175.
Esmadi FT,.Kharoaf MA and Attiyat M S (1991) Sequential Atomic Absorption Spectrometric Determination of Chloride and Iodide in A Flow System Using an On-Line Preconcentration Technique. Analyst 116: 353.
Koh T, Ono M and Makino I (1989) Spectrophotometric Determination of Iodide at the 10–6 mol L–1 Level by Solvent Extraction with Methylene Blue. Analyst 13:945-948.
Niazi S B, Mozammil M (1991) Spectrophotometric Determination of Traces of Iodide by Liquid-Liquid Extraction of Brilliant Green-Iodide Ion Pair. Anal Chim Acta 252: 115.
Chand P, Narayana B (2006) A Highly Sensitive Spectrophotometric Method for the Determination of Iodate Using Leuco Xylene Cyanol FF. Acta Chim Slov 53: 77.
Narayana B, Cherian T (2005) A Facile Spectrophotometric Method for The Determination of Iodate in Table Salt using New Chromogenic Reagents, South African J Chem 60: 8-10.
Cherian T, Narayana B (2005) Rapid Spectrophotometric Determination of Trace Amounts of Chromium Using Variamine Blue As a Chromogenic Reagent. J Braz Chem Soc 16(2): 197-201.
Bhattacharyya SN and Chetia PK (1967) Spectrophotometric Determination of Periodate in Presence of Iodate Using Aluminum Hydroxide. Anal Chem 19: 369.
Kamburova M (1992) Triphenyltetrazolium chloride for determination of iodate and Periodate, Talanta 39:987.
El-Shahawi M.S and Al-Hashimi FA (1996) Spectrophotometric Determination of Periodate or Iodate Ions by Liquid-Liquid Extraction as an Ion-Pair Using Tetramethylammonium Iodide. Talanta 43: 2037.
Harez A and Bashir W (1985) p-Phenylenediamine as a Chromogen for Iodate and Periodate in Aqueous Solution. Microchem J 31: 375.
Callejon M and Munoz J (1986) Spectrophotometric Determination of Periodate with the Bisthiosemicarbazones of Phthalimide and 1, 3-Indandione. Microchem J 34: 83.
El-Shahawi M.S (1997) Extraction Equilibrium of The Ion-Associate of Periodate with Amiloride Hydrochloride and Simultaneous Spectrophotometric Determination of Periodate and Iodate by Liquid-Liquid Extraction. Anal Chim Acta 356: 85.
25. Balasubramanian N and Maheswari V (1996) Indirect spectrophotometric determination of chromium, Journal of AOAC International 79(4).
Maheswari V and Balasubramanian N (1997) Chemical Amplification Method for the Determination of Chromium at Trace Levels. Analusis 25: 2.
Haree ZAK (1985) p-Phenylenediamine as A Chromogen for Iodate and Periodate In Aqueous Solution. Microchem J 31: 375.
Rammler DH, Bilton R, Haugland R and Parkinson C (1973) Hydrolytic Enzyme Substrates. II. A New Method for Measuring Periodate, Anal. Biochem 52:198.
Verma KK, Gupta DS Sangh SK and Jain A (1987) Spectrophotometric Determination of Periodate With Amodiaquine Dihydrochloride and Its Application to the Indirect Determination of Some Organic Compounds via the Malaprade Reaction. Analyst 112: 1519.
Indian Standard Specification for Iodized Salt IS 7224:1985 (Bureau of Indian Standards, New Delhi, 1986).
Bhatty MK and Townshend A (1971) Spectrophotometric Determination of Small Amounts of Nitrate and Nitrite by Conversion to Nitrotoluene and Extraction Into Toluene. Anal Chim Acta 56: 55.