Spectrophotometric Method for Determination of Trimetazidine in Formulation Using Chloranil as Chromogenic Agent
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Department of Chemical Engineering, Hanseo University; 360 Daegok-ri, Haemi-myun, Season - 360-706, Chungcheongnam-do, South Korea
Department of Nano Technology, Biotechnology centre, Anna University of Technology, Jothipuram,Coimbatore-641047,Tami Nadu, India
Mani Ganesh   

Department of Chemical Engineering, Hanseo University; 360 Daegok-ri, Haemi-myun, Season - 360-706, Chungcheongnam-do, South Korea
Publication date: 2017-10-14
Eurasian J Anal Chem 2011;6(1):22–30
A new, simple, selective and more reproducible spectrophotometric method for the determination of trimetazidine (TMZ), a piperizinyl antianginal has been developed and validated. This method was based on the formation of a blue colored chromogen between trimetazidine and chloranil acetaldehyde reagent in DMSO. The chromogen exhibit maximum absorbance at 627 nm with molar absorptivity of 5.205 x 104 lit mol-1 cm-1 in methanol. Beer’s law was found to be obeyed in the concentration range of 30 - 65 μg mL-1 with linear regression of 0.9984, while the percentage recovery and the limit of detection 98.81-99.91%, 5.5 μg mL-1 respectively. From the percentage recovery and placebo studies it was concluded that there was no interference of additives during the estimation. This shows the suitability of this method for the routine analysis of the trimetazidine in tablet formulation.
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