Stability Indicating Spectrophotometric Methods for Simultaneous Determination of Losartan Potassium and Hydrochlorothiazide in Pharmaceuticals
 
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1
Department of Analytical Chemistry, PDM College of Pharmacy, Bahadurgarh-124507, India
2
National Institute of Pharmaceutical Education and Research, Ahmedabad, Gujarat, India
CORRESPONDING AUTHOR
Permender Rathee   

Department of Analytical Chemistry, PDM College of Pharmacy, Bahadurgarh-124507, India
Publication date: 2017-10-06
 
Eurasian J Anal Chem 2009;4(1):98–109
 
KEYWORDS
ABSTRACT
Two new stability indicating UV-Spectrophotometric methods have been described for the simultaneous assay of Losartan Potassium and Hydrochlorothiazide in bulk drug and in tablet dosage forms using 0.01 N HCl as the solvent. Method A is based on simultaneous equation or Vierodt’s method and Method B is Q-analysis or Q-absorbance ratio method. The lmax values for Losartan Potassium and Hydrochlorothiazide in the solvent medium were found to be 227.4 nm, 270.4 nm and 256.4 nm, 270.4 nm for Method A and Method B respectively. The systems obey Beer’s law in the range of 2.02-22.22 mg mL-1, 3.03-27.27 mg mL-1 and 5.05-50.50 mg mL-1, 3.03-27.27 mg mL-1 for Losartan Potassium and Hydrochlorothiazide for Method A and Method B respectively. Repeatability, Intra-day and interday precision were found to be 0.202 and 0.670, 0.566-1.31, 0.608- 1.35 for Method A and 0.989 and 0.586, 0.561-1.30, 0.602- 1.33 for Method B. No interference was observed from common tablet adjuvants. t –test and F-test have been applied for the recovery studies of the two methods. The methods were successfully applied to the assay of Losartan Potassium and Hydrochlorothiazide in tablet formulations
 
REFERENCES (23)
1.
USP30–NF25, US Pharmacopeial Convention Inc.: Rockwille, USA, 2499.
 
2.
Martindale (2005) The Complete Drug Reference; 34th ed.; Pharmaceutical Press, USA, 933.
 
3.
Hassan J, Effat S, Hassan F and Mehdi A (2003) A High-Performance Liquid Chromatographic Assay for the Determination of Losartan in Plasma. Iranian Journal of Pharmacology & Therapeutics 2:18-21.
 
4.
Sivakumar T, Venkatesan P, Manavalan R and Valliappan K (2007) Development of a HPLC method for the simultaneous determination of losartan potassium and atenolol in tablets. Indian Journal of pharmaceutical Sciences 69 (1): 154-157.
 
5.
Carlucci G, Palumbo G, Mazzeo P and Quaglia MG (2000) Simultaneous determination of losartan and hydrochlorothiazide in tablets by high-performance liquid chromatography. J. Pharm. Biomed. Anal. 23: 185–189.
 
6.
Hertzog DL, McCafferty JF, Fang X, Tyrell RJ and Reed RA (2002) Development and validation of a stability-indicating HPLC method for the simultaneous determination of Losartan potassium, hydrochlorothiazide, and their degradation products. J. Pharm. Biomed. Anal. 30: 747–760.
 
7.
Mahadik K R, Aggrawal H and Kaul N (2004) Simultaneous HPTLC Estimation of Amlodipine Besylate and Losartan Potassium in Tablet Dosage Form. 41 (1): 32-36.
 
8.
Gandhimathi M, Ravi T, Ninan A and Varghese A (2004) RP HPLC Determination of Losartan Potassium and Ramipril in Tablets. Indian drugs 41 (1): 36-40.
 
9.
Ardal D (2005) A New Application of Chemometric Techniques to HPLC Data for the Simultaneous Analysis of a Two-Component Mixture. J. of Liquid Chromatography and related techniques 28 (14): 2179-2194.
 
10.
Argekar AP and Sawant JG (2000) A Gradient Reversed Phase High Performance Liquid Chromatography Method for Simultaneous Determination of Hydrochlorothiazide (HCT) and Losartan Potassium (LOS) from Tablets. Anal. Lett. 33: 869– 880.
 
11.
Baing M M, Vaidya V V, Sane R T, Menon S N and Dalvi K (2006) Simultaneous RP-LC Determination of Losartan Potassium, Ramipril, and Hydrochlorothiazide in Pharmaceutical Preparations. Chromatographia 64 (5-6): 293-296.
 
12.
Erk N (2001) Analysis of binary mixtures of losartan potassium and hydrochlorothiazide by using high performance liquid chromatography, ratio derivative spectrophotometric and compensation technique. J. of Pharm. Biomed. Analysis 24 (4): 603-611.
 
13.
Shah S A, Rathod I S, Suhagia B N, Savale S S and Patel J B (2001) Simultaneous Determination of Losartan and Hydrochlorothiazide in Combined Dosage Forms by First-Derivative Spectroscopy and High-Performance Thin-Layer Chromatography. J. AOAC 84 (6): 1715-1723.
 
14.
Bebawy L I, Abbas S S, Fattah L A and Refaat H H (2005) Application of firstderivative, ratio derivative spectrophotometry, TLC-densitometry and spectrofluorimetry for the simultaneous determination of telmisartan and hydrochlorothiazide in pharmaceutical dosage forms and plasma. Farmaco II 60 (10): 859-867.
 
15.
Mehdi Ansari, Maryam K, Mehdi B and Hassan J (2004) Derivative Spectrophotometric Method for Determination of Losartan in Pharmaceutical Formulations. Iranian Journal of Pharmacology and Therapeutics 3 (1): 21-25.
 
16.
Erk N (2003) Three new spectrophotometric methods applied to the simultaneous determination of hydrochlorothiazide and irbesartan. Pharmazie 58 (8): 543-548.
 
17.
Zhao Z, Wang Q, Tsai EW, Qin XZ and Ip D (1999) Identification of losartan degradates in stressed tablets by LC-MS and LC-MS/MS. J. Pharm. Biomed. Anal. 20 (1-2): 129–136.
 
18.
Hillaert S and Vander B W (2003) Simultaneous determination of hydrochlorothiazide and several angiotensin-II-receptor antagonists by capillary electrophoresis. J. Pharm. Biomed. Anal. 31 (2): 329–339.
 
19.
Quaglia MG, Donati E, Carlucci G, Mazzeo P and Fanali S (2002) Determination of losartan and hydrochlorothiazide in tablets by CE and CEC. J. Pharm. Biomed. Anal. 29 (6): 981–987.
 
20.
Stability testing guidelines (2003) stability testing of new drug substances and products, ICH Q1A (R2), ICH Steering Committee.
 
21.
Green J M (1996) A practical guide to analytical method validation, Anal. Chem. News & Features, 305A/309A.
 
22.
Mend H J (2003) Vogel’s, “Textbook of Quantitative Chemical Analysis”; 6th Ed. Pearson education (Singapore) Pvt. Ltd., Indian branch, Delhi, 3-8, 630.
 
23.
I.C.H. Harmonized Tripartite Guidelines (1994 & 1996) Text on Validation of Analytical Procedures Q2A & Q2B.
 
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