A field investigation was conducted on clay soil of Department of Agronomy, experimental farm at Annamalai University during 2014 to study the response of deshi cotton hybrid to different plant densities and nitrogen levels. The nine treatments comprises of three spacings and three nitrogen levels. The experiment was laid out in factorial randomized block design with three replications. On the basis of results obtained from present investigation it was the lowest plant spacing (90 x 60 cm) that gave more seed cotton yield 2670.40 kg/ha over its highest levels of spacing. Application of 80 kg N/ha significantly gave more seed cotton yield 2834.40 kg/ha over 60 kg N/ha and on par with 100 kg N/ha.