Synthesis of Self-Assembled Hydroxyapatite Membrane Using Bio-Template with Surface Modification
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Universitas Islam Negeri Sumatera Utara
Bandung Institute of Technology
Bandung Institute of Technology,
Online publication date: 2018-07-24
Publication date: 2018-07-24
Eurasian J Anal Chem 2018;13(4):emEJAC04338
Many researches in tissue engineering have been conducted continuously to develop the proper synthetic materials for tissue and organ substitute materials. At this research, the synthesis of hydroxyapatite membrane for bone scaffold application has been done successfully by self assembly method using eggshell membrane with surface modification as a template. By this method, the pores obtained will be able to resemble the cells microenvironment thus can enhance interface interaction and support cell proliferation. Before incubating in modified Simulated Body Fluid (SBF) Kokubo solution, eggshell membrane surface is modified using alginate and chitosan solutions to increase the number of hydroxyapatite particles bonded due to enhance the bioactivity of scaffold. The Scanning Electron Microscopy (SEM) result showed that hydroxyapatite membrane has been formed on scaffold’s surface where the diameter of fibers had thickened to 2.035, 2.591 and 1.408 μm and particle size of 1.021, 0.934 and 0.895 μm for 6, 9 and 12 days incubation time respectively. It confirmed that the number of hydroxyapatite grown increases along with the incubation time. These results are in good agreement with FT-IR analysis that indicates the presence of PO43- functional group on the scaffolds.