A simple, rapid, and precise method is developed for the assay determination of tranexamic acid (Tr) in pharmaceutical dosage form. The method was developed with HPLC, ELS detector and Alltima cyano 100A (250 x 4.6 mm, 5µ) column, the mobile phase composed of aqueous solution of 0.05% v/v trifluoroacetic acid and acetonitrile (60:40 v/v, pH 2.85). The instrumental settings are flow rate of 0.5 mL min^-1, column temperature at 30⁰C and detector drift tube temperature set at 97⁰C, a detector nitrogen gas flow set at 2.6 SLPM, gain 1 and 5 µL sample injection volume. Theoretical plates were 20007. Tailing factor was 1.38. The described method shows excellent linearity over a range of 50 to 150% of assay concentration. The correlation coefficient was 0.9987. The relative standard deviation of six measurements for peak area and retention time less than 2% and 0.5% respectively. The proposed method was found to be suitable and accurate for assay determination of Tr in pharmaceutical preparations.