Stability Indicating Spectrophotometric Methods for Simultaneous Determination of Losartan Potassium and Hydrochlorothiazide in Pharmaceuticals

Permender Rathee, Sushila Rathee, Dharmender Rathee, Hema Chaudhary

Abstract


Two new stability indicating UV-Spectrophotometric methods have been described for the simultaneous assay of Losartan Potassium and Hydrochlorothiazide in bulk drug and in tablet dosage forms using 0.01 N HCl as the solvent. Method A is based on simultaneous equation or Vierodt’s method and Method B is Q-analysis or Q-absorbance ratio method.  The lmax values for Losartan Potassium and Hydrochlorothiazide in the solvent medium were found to be 227.4 nm, 270.4 nm and 256.4 nm, 270.4 nm for Method A and Method B respectively. The systems obey Beer’s law in the range of 2.02-22.22 mg mL-1, 3.03-27.27 mg mL-1 and 5.05-50.50 mg mL-1, 3.03-27.27 mg mL-1   for Losartan Potassium and Hydrochlorothiazide for Method A and Method B respectively. Repeatability, Intra-day and interday precision were found to be 0.202 and 0.670, 0.566-1.31, 0.608- 1.35 for Method A and 0.989 and 0.586, 0.561-1.30, 0.602- 1.33 for Method B. No interference was observed from common tablet adjuvants. t –test and F-test have been applied for the recovery studies of the two methods. The methods were successfully applied to the assay of Losartan Potassium and Hydrochlorothiazide in tablet formulations

Keywords


Losartan Potassium; Hydrochlorothiazide; UV Spectrophotometry; Stability Indicating

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