A simple, rapid, reliable and accurate HPTLC method has been developed for the quantitative determination of Donepezil HCl in bulk and tablets. Various aliquots of this sample solution were spotted automatically by means of camag (Muttenz, Switzerland) Linomat 5 applicator on Merck HPTLC plates (0.2 mm thickness) precoated with silica gel 60 F₂₅₄ on aluminium sheet as stationary phase pre washed with methanol using Methanol: Chloroform (8:2 v/v) as mobile phase. The spots were scanned at 254 nm. The R_f value of Donepezil HCl was 0.54 ± 0.02. Calibration curves were linear in the range of 200-1000 ng/spot. The limit of detection and limit of quantification were found to be 120 ng/spot and 165 ng/spot respectively. The suitability of this method for the quantitative determination of compound was proved by validation in accordance with requirements of pharmaceutical regulatory standards.